Detailed information of TA system TA050127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 36014..36576
• Replicon: plasmid pXF64-HB
• Accession: NZ_CP009886
• Organism: Xylella fastidiosa strain Hib4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: XFHB_RS13930
• Protein ID: WP_088578659.1
• Coordinates: 36014..36310 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: XFHB_RS13935
• Protein ID: WP_088578660.1
• Coordinates: 36298..36576 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFHB_RS13905	32301..32600	-	300	WP_157293288.1	mobilization protein	-
XFHB_RS13910	32842..33276	+	435	WP_088578655.1	hypothetical protein	-
XFHB_RS13915	33290..33970	+	681	WP_088578656.1	AAA family ATPase	-
XFHB_RS13920	33963..34655	+	693	WP_088578657.1	hypothetical protein	-
XFHB_RS13925	34805..35902	-	1098	WP_088578658.1	hypothetical protein	-
XFHB_RS13930	36014..36310	-	297	WP_088578659.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XFHB_RS13935	36298..36576	-	279	WP_088578660.1	CopG family ribbon-helix-helix protein	Antitoxin
XFHB_RS13940	36858..37142	+	285	WP_088578661.1	hypothetical protein	-
XFHB_RS13945	37252..38493	+	1242	WP_157293290.1	hypothetical protein	-
XFHB_RS13950	38614..39192	+	579	WP_088578662.1	recombinase family protein	-
XFHB_RS13955	39125..39433	-	309	WP_088578663.1	hypothetical protein	-
XFHB_RS13960	39592..40311	-	720	WP_088578664.1	DUF3560 domain-containing protein	-
XFHB_RS13970	41051..41329	-	279	WP_088578665.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64251	64251
	-	flank	IS/Tn	-	-	38614..39192	578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11284.93 Da
• Isoelectric Point: 10.0493

>T50127 WP_088578659.1 NZ_CP009886:c36310-36014 [Xylella fastidiosa]
MPRVIFAPEAIRNIQQLRKFLYPKNADAAKRAGEAIIRGTRMLGAQPRIGRPVDDLHDEYREWLIDFGDSGYVARYRIDG
DTVTILAVRHQKEVGYTR

Nucleotide

• Length: 297 bp

>T50127 NZ_CP009886:c36310-36014 [Xylella fastidiosa]
ATGCCACGAGTGATCTTCGCTCCAGAAGCCATCCGGAACATTCAACAATTGCGGAAGTTCCTGTATCCGAAGAACGCCGA
TGCCGCAAAGCGGGCCGGTGAAGCCATCATCCGGGGTACCCGGATGCTCGGAGCGCAGCCACGTATCGGCCGACCGGTTG
ATGACCTGCACGATGAGTACCGGGAATGGCTAATTGACTTCGGAGATAGCGGTTATGTGGCCCGCTACCGCATTGATGGC
GATACCGTCACGATCTTGGCTGTTCGACACCAGAAGGAAGTAGGATACACCAGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10872.22 Da
• Isoelectric Point: 5.2160

>AT50127 WP_088578660.1 NZ_CP009886:c36576-36298 [Xylella fastidiosa]
MGMATSIKLDDEMKGRVQHLADVRRRTSHWIMREAITQYVEREEKREAFRQDTLKAWEEFQATGLHVTTEEVEKWLSSWG
TENELPAPVCHE

Nucleotide

• Length: 279 bp

>AT50127 NZ_CP009886:c36576-36298 [Xylella fastidiosa]
ATGGGCATGGCAACCTCCATCAAGCTTGATGATGAGATGAAAGGACGTGTGCAGCATCTGGCCGATGTTCGCCGCCGCAC
TTCGCACTGGATCATGCGCGAGGCGATCACGCAGTACGTTGAGCGTGAGGAAAAGCGTGAGGCGTTTCGGCAGGACACGT
TGAAGGCATGGGAAGAGTTTCAGGCGACCGGCCTGCACGTGACGACCGAAGAAGTCGAGAAGTGGCTTTCGAGTTGGGGC
ACTGAAAACGAATTGCCAGCGCCCGTATGCCACGAGTGA