Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3713323..3713966 Replicon chromosome
Accession NZ_CP009876
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH31

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag KPNIH31_RS18300 Protein ID WP_032433387.1
Coordinates 3713323..3713739 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag KPNIH31_RS18305 Protein ID WP_001261276.1
Coordinates 3713736..3713966 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH31_RS18275 3708755..3709246 - 492 WP_032433378.1 hypothetical protein -
KPNIH31_RS18280 3709277..3709795 - 519 WP_045326794.1 hypothetical protein -
KPNIH31_RS18285 3709854..3710102 - 249 WP_032433382.1 hypothetical protein -
KPNIH31_RS18290 3710421..3711992 + 1572 WP_032433383.1 ATP-binding protein -
KPNIH31_RS18295 3712187..3713167 - 981 WP_032433385.1 hypothetical protein -
KPNIH31_RS18300 3713323..3713739 - 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
KPNIH31_RS18305 3713736..3713966 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KPNIH31_RS18310 3714271..3715086 + 816 WP_032433388.1 hypothetical protein -
KPNIH31_RS18320 3715614..3717854 - 2241 WP_045326788.1 NTPase -
KPNIH31_RS18325 3717857..3718944 - 1088 Protein_3510 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3713323..3730396 17073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T50108 WP_032433387.1 NZ_CP009876:c3713739-3713323 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T50108 NZ_CP009876:c3713739-3713323 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT50108 WP_001261276.1 NZ_CP009876:c3713966-3713736 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT50108 NZ_CP009876:c3713966-3713736 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References