Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3694407..3695143 Replicon chromosome
Accession NZ_CP009876
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH31

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag KPNIH31_RS18195 Protein ID WP_032433360.1
Coordinates 3694407..3694889 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KPNIH31_RS18200 Protein ID WP_003026799.1
Coordinates 3694877..3695143 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH31_RS28265 3689875..3690084 + 210 WP_144340089.1 molecular chaperone -
KPNIH31_RS18170 3690106..3691074 + 969 WP_076026135.1 IS5-like element IS903B family transposase -
KPNIH31_RS28270 3691172..3691696 + 525 WP_071889011.1 molecular chaperone -
KPNIH31_RS18180 3691700..3692278 + 579 WP_032432061.1 type 1 fimbrial protein -
KPNIH31_RS18185 3692290..3693234 + 945 WP_077254249.1 fimbrial protein -
KPNIH31_RS18190 3693447..3694046 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
KPNIH31_RS18195 3694407..3694889 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
KPNIH31_RS18200 3694877..3695143 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KPNIH31_RS18205 3695456..3696019 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
KPNIH31_RS18210 3696029..3696661 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
KPNIH31_RS18215 3696679..3697683 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
KPNIH31_RS18220 3697667..3698329 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
KPNIH31_RS18225 3698358..3699497 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3685579..3701585 16006
- flank IS/Tn - - 3690151..3691074 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T50107 WP_032433360.1 NZ_CP009876:c3694889-3694407 [Klebsiella pneumoniae subsp. pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T50107 NZ_CP009876:c3694889-3694407 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT50107 WP_003026799.1 NZ_CP009876:c3695143-3694877 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT50107 NZ_CP009876:c3695143-3694877 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References