Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1289832..1290451 Replicon chromosome
Accession NZ_CP009876
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH31

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KPNIH31_RS06315 Protein ID WP_002892050.1
Coordinates 1289832..1290050 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KPNIH31_RS06320 Protein ID WP_002892066.1
Coordinates 1290077..1290451 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH31_RS06280 1285875..1286138 + 264 WP_032432663.1 type B 50S ribosomal protein L31 -
KPNIH31_RS06285 1286138..1286278 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KPNIH31_RS06290 1286275..1286973 - 699 WP_023287311.1 GNAT family N-acetyltransferase -
KPNIH31_RS06295 1287074..1288525 + 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
KPNIH31_RS06300 1288500..1288970 - 471 WP_020802585.1 YlaC family protein -
KPNIH31_RS29785 1288991..1289131 + 141 WP_004147370.1 hypothetical protein -
KPNIH31_RS06310 1289103..1289669 - 567 WP_002892030.1 maltose O-acetyltransferase -
KPNIH31_RS06315 1289832..1290050 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
KPNIH31_RS06320 1290077..1290451 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KPNIH31_RS06325 1290937..1294083 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KPNIH31_RS06330 1294106..1295299 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T50097 WP_002892050.1 NZ_CP009876:c1290050-1289832 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T50097 NZ_CP009876:c1290050-1289832 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT50097 WP_002892066.1 NZ_CP009876:c1290451-1290077 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT50097 NZ_CP009876:c1290451-1290077 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References