Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35984..36627 Replicon plasmid pKpQIL-531
Accession NZ_CP009875
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH30

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KPNIH30_RS27375 Protein ID WP_001044770.1
Coordinates 35984..36400 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KPNIH30_RS27380 Protein ID WP_001261282.1
Coordinates 36397..36627 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH30_RS27335 31526..31762 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
KPNIH30_RS27340 31759..32121 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
KPNIH30_RS27345 32139..33833 - 1695 WP_000105636.1 mercury(II) reductase -
KPNIH30_RS27350 33885..34307 - 423 WP_001340589.1 organomercurial transporter MerC -
KPNIH30_RS27355 34343..34453 - 111 Protein_37 mercuric transport protein periplasmic component -
KPNIH30_RS27360 34469..35166 - 698 Protein_38 IS1 family transposase -
KPNIH30_RS27370 35200..35910 - 711 WP_004152334.1 ATP-binding protein -
KPNIH30_RS27375 35984..36400 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KPNIH30_RS27380 36397..36627 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KPNIH30_RS27385 36584..37045 + 462 WP_072093212.1 hypothetical protein -
KPNIH30_RS27395 37532..37840 + 309 WP_004152336.1 hypothetical protein -
KPNIH30_RS27400 37868..38197 + 330 WP_004152337.1 hypothetical protein -
KPNIH30_RS27405 38223..38621 + 399 WP_004171440.1 hypothetical protein -
KPNIH30_RS27410 38628..38960 + 333 WP_004152339.1 hypothetical protein -
KPNIH30_RS27415 38960..39742 + 783 WP_004152340.1 site-specific integrase -
KPNIH30_RS29130 40634..40864 - 231 WP_011977773.1 hypothetical protein -
KPNIH30_RS29135 40956..41429 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A - 1..113639 113639
- flank IS/Tn - - 41549..42817 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T50092 WP_001044770.1 NZ_CP009875:c36400-35984 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T50092 NZ_CP009875:c36400-35984 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT50092 WP_001261282.1 NZ_CP009875:c36627-36397 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT50092 NZ_CP009875:c36627-36397 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References