Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 152913..153823 Replicon plasmid pKPN-80a
Accession NZ_CP009865
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH29

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag KPNIH29_RS27550 Protein ID WP_004144067.1
Coordinates 152913..153383 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A7H4PRG4
Locus tag KPNIH29_RS27555 Protein ID WP_032413443.1
Coordinates 153380..153823 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH29_RS27515 148186..148533 - 348 WP_023288267.1 IS66 family insertion sequence element accessory protein TnpB -
KPNIH29_RS27520 148530..148868 - 339 WP_040120139.1 IS66 family insertion sequence hypothetical protein -
KPNIH29_RS27525 148961..149419 + 459 Protein_161 DDE-type integrase/transposase/recombinase -
KPNIH29_RS27530 149624..149935 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin -
KPNIH29_RS27535 149932..150351 + 420 WP_011251286.1 helix-turn-helix domain-containing protein -
KPNIH29_RS27540 150498..151466 + 969 WP_077255815.1 IS5 family transposase -
KPNIH29_RS29125 151525..151971 + 447 Protein_165 transposase -
KPNIH29_RS27545 152543..152803 + 261 WP_017901409.1 hypothetical protein -
KPNIH29_RS27550 152913..153383 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
KPNIH29_RS27555 153380..153823 - 444 WP_032413443.1 DUF2384 domain-containing protein Antitoxin
KPNIH29_RS29130 153923..154120 - 198 Protein_169 DUF4113 domain-containing protein -
KPNIH29_RS27560 154162..155130 - 969 WP_077255817.1 IS5 family transposase -
KPNIH29_RS29135 155200..155406 - 207 Protein_171 transposase -
KPNIH29_RS29140 156396..156509 + 114 Protein_172 transposase -
KPNIH29_RS29435 156930..157043 + 114 WP_077253754.1 IS3 family transposase -
KPNIH29_RS27565 157135..158733 - 1599 WP_077255438.1 sensor domain-containing diguanylate cyclase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..159360 159360
- inside IScluster/Tn - - 142423..155397 12974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre

(16-147)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T50073 WP_004144067.1 NZ_CP009865:c153383-152913 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T50073 NZ_CP009865:c153383-152913 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCTTCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16508.82 Da        Isoelectric Point: 10.3013

>AT50073 WP_032413443.1 NZ_CP009865:c153823-153380 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT50073 NZ_CP009865:c153823-153380 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACTGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A7H4PRG4

References