Detailed information of TA system TA050070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 64093..64615
• Replicon: plasmid pKPN-80a
• Accession: NZ_CP009865
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPNIH29

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: KPNIH29_RS27110
• Protein ID: WP_004187019.1
• Coordinates: 64093..64377 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: KPNIH29_RS27115
• Protein ID: WP_004187025.1
• Coordinates: 64367..64615 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPNIH29_RS27085	59168..60091	-	924	WP_040120118.1	cation diffusion facilitator family transporter	-
KPNIH29_RS29080	60352..60672	+	321	WP_071888966.1	metalloregulator ArsR/SmtB family transcription factor	-
KPNIH29_RS27095	60718..62007	+	1290	WP_040120120.1	arsenite efflux transporter membrane subunit ArsB	-
KPNIH29_RS27100	62020..62448	+	429	WP_035897523.1	glutaredoxin-dependent arsenate reductase	-
KPNIH29_RS27105	62734..64077	+	1344	WP_040120121.1	ISNCY family transposase	-
KPNIH29_RS27110	64093..64377	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KPNIH29_RS27115	64367..64615	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
KPNIH29_RS27120	65124..66092	-	969	WP_077255813.1	IS5 family transposase	-
KPNIH29_RS27125	66419..67231	-	813	WP_040120122.1	ribokinase	-
KPNIH29_RS27130	67244..68218	-	975	WP_003032095.1	SIS domain-containing protein	-
KPNIH29_RS27135	68223..69008	-	786	WP_003032097.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..159360	159360
	-	flank	IS/Tn	-	-	65124..66092	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T50070 WP_004187019.1 NZ_CP009865:c64377-64093 [Klebsiella pneumoniae subsp. pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T50070 NZ_CP009865:c64377-64093 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT50070 WP_004187025.1 NZ_CP009865:c64615-64367 [Klebsiella pneumoniae subsp. pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT50070 NZ_CP009865:c64615-64367 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4