Detailed information of TA system TA050069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 51205..51956
• Replicon: plasmid pKPN-80a
• Accession: NZ_CP009865
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPNIH29

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KPNIH29_RS27035
• Protein ID: WP_016946197.1
• Coordinates: 51474..51956 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: KPNIH29_RS27030
• Protein ID: WP_004902250.1
• Coordinates: 51205..51483 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPNIH29_RS27010	46986..47870	-	885	WP_004186900.1	hypothetical protein	-
KPNIH29_RS27015	49124..49582	+	459	WP_014386535.1	hypothetical protein	-
KPNIH29_RS27020	50422..50763	+	342	WP_032437747.1	hypothetical protein	-
KPNIH29_RS27025	50871..51083	+	213	WP_016946198.1	hypothetical protein	-
KPNIH29_RS27030	51205..51483	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
KPNIH29_RS27035	51474..51956	+	483	WP_016946197.1	GNAT family N-acetyltransferase	Toxin
KPNIH29_RS27040	52047..52744	+	698	Protein_59	IS1 family transposase	-
KPNIH29_RS27045	53158..53406	+	249	Protein_60	IS5/IS1182 family transposase	-
KPNIH29_RS27050	53757..53945	+	189	Protein_61	hypothetical protein	-
KPNIH29_RS27055	54052..55074	+	1023	Protein_62	IS630 family transposase	-
KPNIH29_RS27060	55172..56140	-	969	WP_077255812.1	IS5 family transposase	-
KPNIH29_RS30585	56158..56931	-	774	WP_187524108.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..159360	159360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17724.53 Da
• Isoelectric Point: 8.9130

>T50069 WP_016946197.1 NZ_CP009865:51474-51956 [Klebsiella pneumoniae subsp. pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWSGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T50069 NZ_CP009865:51474-51956 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGATGTCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGCAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTTATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGTCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATTGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT50069 WP_004902250.1 NZ_CP009865:51205-51483 [Klebsiella pneumoniae subsp. pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT50069 NZ_CP009865:51205-51483 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACACCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3