Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4450622..4451279 Replicon chromosome
Accession NZ_CP009863
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH29

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag KPNIH29_RS22100 Protein ID WP_002916310.1
Coordinates 4450622..4451032 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag KPNIH29_RS22105 Protein ID WP_002916312.1
Coordinates 4451013..4451279 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH29_RS22080 4446622..4448355 - 1734 WP_004149758.1 single-stranded-DNA-specific exonuclease RecJ -
KPNIH29_RS22085 4448361..4449074 - 714 WP_004174456.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KPNIH29_RS22090 4449097..4449993 - 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
KPNIH29_RS22095 4450094..4450615 + 522 WP_002916308.1 flavodoxin FldB -
KPNIH29_RS22100 4450622..4451032 - 411 WP_002916310.1 protein YgfX Toxin
KPNIH29_RS22105 4451013..4451279 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
KPNIH29_RS22110 4451525..4452508 + 984 WP_038435686.1 tRNA-modifying protein YgfZ -
KPNIH29_RS22115 4452659..4453318 - 660 WP_002916317.1 hemolysin III family protein -
KPNIH29_RS22120 4453482..4453793 - 312 WP_004144734.1 N(4)-acetylcytidine aminohydrolase -
KPNIH29_RS22125 4453843..4454571 + 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
KPNIH29_RS22130 4454690..4456123 + 1434 WP_038435687.1 6-phospho-beta-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T50065 WP_002916310.1 NZ_CP009863:c4451032-4450622 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T50065 NZ_CP009863:c4451032-4450622 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT50065 WP_002916312.1 NZ_CP009863:c4451279-4451013 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50065 NZ_CP009863:c4451279-4451013 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References