Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1265522..1266141 Replicon chromosome
Accession NZ_CP009863
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH29

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KPNIH29_RS06135 Protein ID WP_002892050.1
Coordinates 1265522..1265740 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KPNIH29_RS06140 Protein ID WP_002892066.1
Coordinates 1265767..1266141 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH29_RS06100 1261569..1261832 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
KPNIH29_RS06105 1261832..1261972 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KPNIH29_RS06110 1261969..1262667 - 699 WP_004177238.1 GNAT family N-acetyltransferase -
KPNIH29_RS06115 1262768..1264219 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KPNIH29_RS06120 1264194..1264664 - 471 WP_002892026.1 YlaC family protein -
KPNIH29_RS30125 1264685..1264825 + 141 WP_004147370.1 hypothetical protein -
KPNIH29_RS06130 1264797..1265363 - 567 WP_002892030.1 maltose O-acetyltransferase -
KPNIH29_RS06135 1265522..1265740 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
KPNIH29_RS06140 1265767..1266141 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KPNIH29_RS06145 1266627..1269773 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KPNIH29_RS06150 1269796..1270989 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T50055 WP_002892050.1 NZ_CP009863:c1265740-1265522 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T50055 NZ_CP009863:c1265740-1265522 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT50055 WP_002892066.1 NZ_CP009863:c1266141-1265767 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT50055 NZ_CP009863:c1266141-1265767 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References