Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 532021..532537 Replicon chromosome
Accession NZ_CP009863
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH29

Toxin (Protein)


Gene name relE Uniprot ID R4YAY3
Locus tag KPNIH29_RS02635 Protein ID WP_004178374.1
Coordinates 532253..532537 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag KPNIH29_RS02630 Protein ID WP_002886901.1
Coordinates 532021..532263 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH29_RS02615 528048..528788 + 741 WP_002886899.1 KDGP aldolase family protein -
KPNIH29_RS02620 528855..530010 + 1156 Protein_462 lactonase family protein -
KPNIH29_RS02625 530033..531943 + 1911 WP_032421964.1 BglG family transcription antiterminator -
KPNIH29_RS02630 532021..532263 + 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
KPNIH29_RS02635 532253..532537 + 285 WP_004178374.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KPNIH29_RS02640 532541..533005 - 465 WP_004222151.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
KPNIH29_RS02645 533314..535452 - 2139 WP_004186701.1 anaerobic ribonucleoside-triphosphate reductase -
KPNIH29_RS02655 535809..536552 - 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
KPNIH29_RS30050 536555..536728 - 174 WP_002886906.1 hypothetical protein -
KPNIH29_RS02665 536813..537121 + 309 WP_016947038.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11155.97 Da        Isoelectric Point: 10.3787

>T50053 WP_004178374.1 NZ_CP009863:532253-532537 [Klebsiella pneumoniae subsp. pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T50053 NZ_CP009863:532253-532537 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT50053 WP_002886901.1 NZ_CP009863:532021-532263 [Klebsiella pneumoniae subsp. pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT50053 NZ_CP009863:532021-532263 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6THG1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References