Detailed information of TA system TA050041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 44695..45254
• Replicon: plasmid pECO-824
• Accession: NZ_CP009860
• Organism: Escherichia coli strain ECONIH1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: ECONIH1_RS26645
• Protein ID: WP_000421272.1
• Coordinates: 44695..44970 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: ECONIH1_RS26650
• Protein ID: WP_001387467.1
• Coordinates: 44970..45254 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECONIH1_RS26615	40745..41485	-	741	WP_001066953.1	tyrosine-type recombinase/integrase	-
ECONIH1_RS31490	41606..41788	-	183	WP_042344623.1	hypothetical protein	-
ECONIH1_RS30005	42131..42438	-	308	Protein_41	type II toxin-antitoxin system ParD family antitoxin	-
ECONIH1_RS26635	42931..44100	+	1170	WP_001072355.1	hypothetical protein	-
ECONIH1_RS31495	44133..44261	+	129	Protein_43	IS481 family transposase	-
ECONIH1_RS26640	44296..44589	-	294	WP_001105060.1	hypothetical protein	-
ECONIH1_RS26645	44695..44970	-	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECONIH1_RS26650	44970..45254	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
ECONIH1_RS26655	45859..46611	+	753	WP_000562172.1	IclR family transcriptional regulator	-
ECONIH1_RS26660	46657..47622	-	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
ECONIH1_RS26665	47655..48035	-	381	WP_000710783.1	Rid family detoxifying hydrolase	-
ECONIH1_RS26670	48060..48950	-	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
ECONIH1_RS26675	49183..49377	-	195	WP_000796505.1	hypothetical protein	-
ECONIH1_RS26680	49351..49791	+	441	Protein_52	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / erm(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15	iucA / iucB / iucC / iucD / iutA	1..121385	121385

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T50041 WP_000421272.1 NZ_CP009860:c44970-44695 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T50041 NZ_CP009860:c44970-44695 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT50041 WP_001387467.1 NZ_CP009860:c45254-44970 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT50041 NZ_CP009860:c45254-44970 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880