Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17128..17771 Replicon plasmid pECO-824
Accession NZ_CP009860
Organism Escherichia coli strain ECONIH1

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag ECONIH1_RS26500 Protein ID WP_001034044.1
Coordinates 17355..17771 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag ECONIH1_RS26495 Protein ID WP_001261286.1
Coordinates 17128..17358 (+) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECONIH1_RS26480 12265..12495 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ECONIH1_RS26485 12492..12908 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
ECONIH1_RS26490 12953..16747 - 3795 WP_001144732.1 hypothetical protein -
ECONIH1_RS26495 17128..17358 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ECONIH1_RS26500 17355..17771 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
ECONIH1_RS26505 17846..19411 + 1566 WP_001128474.1 AAA family ATPase -
ECONIH1_RS26510 19396..20418 + 1023 WP_000361402.1 helicase UvrD -
ECONIH1_RS26515 20672..21369 - 698 Protein_20 IS1 family transposase -
ECONIH1_RS26520 21725..22639 + 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / erm(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 iucA / iucB / iucC / iucD / iutA 1..121385 121385
- flank IS/Tn sitABCD - 20672..25174 4502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-129)

Antitoxin

(7-52)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T50040 WP_001034044.1 NZ_CP009860:17355-17771 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T50040 NZ_CP009860:17355-17771 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT50040 WP_001261286.1 NZ_CP009860:17128-17358 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT50040 NZ_CP009860:17128-17358 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829CHW1


Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References