Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 7776..8301 Replicon plasmid pECO-824
Accession NZ_CP009860
Organism Escherichia coli strain ECONIH1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ECONIH1_RS26450 Protein ID WP_001159868.1
Coordinates 7776..8081 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ECONIH1_RS26455 Protein ID WP_000813634.1
Coordinates 8083..8301 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECONIH1_RS26435 3686..4852 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
ECONIH1_RS26440 5440..6195 - 756 WP_024946706.1 replication initiation protein RepE -
ECONIH1_RS26445 6969..7775 - 807 WP_000016982.1 site-specific integrase -
ECONIH1_RS26450 7776..8081 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ECONIH1_RS26455 8083..8301 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ECONIH1_RS26460 8868..9380 + 513 WP_000151784.1 hypothetical protein -
ECONIH1_RS26465 9414..10547 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
ECONIH1_RS26470 10714..11487 - 774 WP_000905949.1 hypothetical protein -
ECONIH1_RS26475 11500..12000 - 501 WP_000528931.1 hypothetical protein -
ECONIH1_RS26480 12265..12495 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ECONIH1_RS26485 12492..12908 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / erm(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 iucA / iucB / iucC / iucD / iutA 1..121385 121385


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T50038 WP_001159868.1 NZ_CP009860:c8081-7776 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T50038 NZ_CP009860:c8081-7776 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT50038 WP_000813634.1 NZ_CP009860:c8301-8083 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT50038 NZ_CP009860:c8301-8083 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References