50032

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4959001..4959498 Replicon chromosome
Accession NZ_CP009859
Organism Escherichia coli strain ECONIH1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECONIH1_RS24605 Protein ID WP_001173343.1
Coordinates 4959325..4959498 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECONIH1_RS24600 Protein ID WP_000398619.1
Coordinates 4959001..4959297 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECONIH1_RS24580 4955730..4956449 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECONIH1_RS24585 4956446..4958077 - 1632 WP_022646455.1 sensor histidine kinase -
ECONIH1_RS24590 4958258..4958488 + 231 WP_001683478.1 (4Fe-4S)-binding protein -
ECONIH1_RS24595 4958500..4958772 + 273 WP_000405647.1 N-acetyltransferase -
ECONIH1_RS24600 4959001..4959297 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECONIH1_RS24605 4959325..4959498 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECONIH1_RS24610 4959617..4961134 - 1518 WP_022646456.1 lysine--tRNA ligase -
ECONIH1_RS24615 4961371..4962828 - 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T50032 WP_001173343.1 NZ_CP009859:4959325-4959498 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T50032 NZ_CP009859:4959325-4959498 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT50032 WP_000398619.1 NZ_CP009859:4959001-4959297 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT50032 NZ_CP009859:4959001-4959297 [Escherichia coli]
ATGGAAGGTAAAAATAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References