Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3377982..3378636 Replicon chromosome
Accession NZ_CP009859
Organism Escherichia coli strain ECONIH1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag ECONIH1_RS16685 Protein ID WP_000244781.1
Coordinates 3377982..3378389 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECONIH1_RS16690 Protein ID WP_000354046.1
Coordinates 3378370..3378636 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECONIH1_RS16665 3373939..3375672 - 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -
ECONIH1_RS16670 3375678..3376388 - 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECONIH1_RS16675 3376413..3377309 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECONIH1_RS16680 3377421..3377942 + 522 WP_001055874.1 flavodoxin FldB -
ECONIH1_RS16685 3377982..3378389 - 408 WP_000244781.1 protein YgfX Toxin
ECONIH1_RS16690 3378370..3378636 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECONIH1_RS16695 3378889..3379869 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
ECONIH1_RS16700 3379946..3380605 - 660 WP_000250269.1 hemolysin III family protein -
ECONIH1_RS16705 3380769..3381080 - 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
ECONIH1_RS16710 3381125..3382558 + 1434 WP_022646090.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T50026 WP_000244781.1 NZ_CP009859:c3378389-3377982 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50026 NZ_CP009859:c3378389-3377982 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50026 WP_000354046.1 NZ_CP009859:c3378636-3378370 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50026 NZ_CP009859:c3378636-3378370 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References