Detailed information of TA system TA050012

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1114534..1115378
• Replicon: chromosome
• Accession: NZ_CP009859
• Organism: Escherichia coli strain ECONIH1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: ECONIH1_RS05645
• Protein ID: WP_000854686.1
• Coordinates: 1114995..1115378 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: ECONIH1_RS05640
• Protein ID: WP_001285602.1
• Coordinates: 1114534..1114914 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECONIH1_RS05605	1110784..1111238	+	455	Protein_1059	hypothetical protein	-
ECONIH1_RS05610	1111317..1111550	+	234	WP_001119717.1	DUF905 family protein	-
ECONIH1_RS05615	1111650..1112468	+	819	WP_001234726.1	DUF945 domain-containing protein	-
ECONIH1_RS05620	1112560..1113045	+	486	WP_000214307.1	antirestriction protein	-
ECONIH1_RS05625	1113061..1113537	+	477	WP_001186726.1	RadC family protein	-
ECONIH1_RS05630	1113600..1113821	+	222	WP_000692298.1	DUF987 domain-containing protein	-
ECONIH1_RS05635	1113840..1114523	+	684	WP_000086768.1	hypothetical protein	-
ECONIH1_RS05640	1114534..1114914	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECONIH1_RS05645	1114995..1115378	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
ECONIH1_RS05650	1115375..1115863	+	489	WP_001054233.1	hypothetical protein	-
ECONIH1_RS05655	1115880..1116077	+	198	WP_000839253.1	DUF957 domain-containing protein	-
ECONIH1_RS05660	1116162..1117004	+	843	WP_001529751.1	DUF4942 domain-containing protein	-
ECONIH1_RS05670	1117495..1117713	-	219	WP_001040187.1	translation initiation factor IF-1	-
ECONIH1_RS05675	1117998..1118702	-	705	WP_022645453.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	iroN / iroE / iroD / iroC / aggD / aafC	1055312..1115378	60066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T50012 WP_000854686.1 NZ_CP009859:1114995-1115378 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T50012 NZ_CP009859:1114995-1115378 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT50012 WP_001285602.1 NZ_CP009859:1114534-1114914 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT50012 NZ_CP009859:1114534-1114914 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG