Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 76627..77366 Replicon plasmid pENT-22e
Accession NZ_CP009855
Organism UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH5

Toxin (Protein)


Gene name tacT Uniprot ID A0A9E6WPH7
Locus tag ECNIH5_RS22310 Protein ID WP_013087269.1
Coordinates 76627..77112 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A9E6WPN8
Locus tag ECNIH5_RS22315 Protein ID WP_007869691.1
Coordinates 77100..77366 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH5_RS22290 (ECNIH5_22290) 71779..72855 + 1077 WP_000227970.1 IS110 family transposase -
ECNIH5_RS25135 72872..73180 + 309 Protein_84 integrase core domain-containing protein -
ECNIH5_RS25140 (ECNIH5_22295) 73224..74562 + 1339 Protein_85 IS3 family transposase -
ECNIH5_RS26085 74775..74906 - 132 WP_255409921.1 hypothetical protein -
ECNIH5_RS22305 (ECNIH5_22305) 75075..76420 - 1346 Protein_87 ISNCY family transposase -
ECNIH5_RS22310 (ECNIH5_22310) 76627..77112 - 486 WP_013087269.1 GNAT family N-acetyltransferase Toxin
ECNIH5_RS22315 (ECNIH5_22315) 77100..77366 - 267 WP_007869691.1 DUF1778 domain-containing protein Antitoxin
ECNIH5_RS22325 (ECNIH5_22325) 78019..79032 + 1014 WP_040119789.1 plasmid replication initiator RepA -
ECNIH5_RS22330 (ECNIH5_22330) 79922..80725 + 804 WP_032664829.1 DUF4225 domain-containing protein -
ECNIH5_RS22335 (ECNIH5_22335) 80703..81026 - 324 WP_040119790.1 hypothetical protein -
ECNIH5_RS22340 (ECNIH5_22340) 81041..81811 - 771 WP_032664827.1 TraX family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..111232 111232


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-146)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17736.45 Da        Isoelectric Point: 9.5181

>T50005 WP_013087269.1 NZ_CP009855:c77112-76627 [Enterobacter cloacae]
VGRVTAPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKRDTKQVVGFYSLATGSVNHVEAMGSLRRNM
PDPIPVIILARLAVDVSYRGQGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYLHHGFKASQTQERTLFLKLP
Q

Download         Length: 486 bp

>T50005 NZ_CP009855:c77112-76627 [Enterobacter cloacae]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10080.46 Da        Isoelectric Point: 4.3837

>AT50005 WP_007869691.1 NZ_CP009855:c77366-77100 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVSDEAAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT50005 NZ_CP009855:c77366-77100 [Enterobacter cloacae]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References