Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4588571..4589187 Replicon chromosome
Accession NZ_CP009854
Organism UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH5

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag ECNIH5_RS21715 Protein ID WP_080298797.1
Coordinates 4588571..4588942 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A9E7BIL4
Locus tag ECNIH5_RS21720 Protein ID WP_023298920.1
Coordinates 4588945..4589187 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH5_RS21700 (ECNIH5_21700) 4586071..4586973 + 903 WP_003861960.1 formate dehydrogenase subunit beta -
ECNIH5_RS21705 (ECNIH5_21705) 4586970..4587605 + 636 WP_006808711.1 formate dehydrogenase cytochrome b556 subunit -
ECNIH5_RS21710 (ECNIH5_21710) 4587602..4588531 + 930 WP_022649835.1 formate dehydrogenase accessory protein FdhE -
ECNIH5_RS21715 (ECNIH5_21715) 4588571..4588942 - 372 WP_080298797.1 type II toxin-antitoxin system VapC family toxin Toxin
ECNIH5_RS21720 (ECNIH5_21720) 4588945..4589187 - 243 WP_023298920.1 ribbon-helix-helix protein, CopG family Antitoxin
ECNIH5_RS21725 (ECNIH5_21725) 4589387..4590307 + 921 WP_038416944.1 alpha/beta hydrolase -
ECNIH5_RS21730 (ECNIH5_21730) 4590316..4591257 - 942 WP_038416946.1 fatty acid biosynthesis protein FabY -
ECNIH5_RS21735 (ECNIH5_21735) 4591302..4591739 - 438 WP_015569909.1 D-aminoacyl-tRNA deacylase -
ECNIH5_RS21740 (ECNIH5_21740) 4591736..4592617 - 882 WP_023298923.1 virulence factor BrkB family protein -
ECNIH5_RS21745 (ECNIH5_21745) 4592611..4593210 - 600 WP_023315056.1 glucose-1-phosphatase -
ECNIH5_RS21750 (ECNIH5_21750) 4593329..4594129 - 801 WP_038416949.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-114)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13612.66 Da        Isoelectric Point: 6.4865

>T50004 WP_080298797.1 NZ_CP009854:c4588942-4588571 [Enterobacter cloacae]
MEHMAVYDTNILIDLFNNHVEAADAIDRTASHRAISLITWMEVMVGARRHGQEAKTAAVMGAFEIIDITREIAERSVLLR
EQHGMKLPDAIILATAQSRSCPLISRNTKDFSGIAGVVSPYHL

Download         Length: 372 bp

>T50004 NZ_CP009854:c4588942-4588571 [Enterobacter cloacae]
ATGGAGCACATGGCTGTGTATGACACCAATATCCTCATCGATCTTTTTAATAACCACGTTGAGGCTGCGGATGCGATCGA
TCGCACAGCTTCGCATCGTGCCATTAGCCTCATCACATGGATGGAAGTAATGGTGGGCGCTCGCAGGCACGGTCAAGAAG
CAAAAACGGCAGCCGTGATGGGGGCCTTTGAAATTATTGATATAACAAGGGAAATTGCTGAAAGAAGCGTATTACTTCGC
GAACAGCACGGGATGAAGCTTCCTGACGCCATTATTCTGGCTACTGCTCAGAGCAGAAGCTGTCCGTTAATTTCGCGCAA
TACGAAAGACTTTTCGGGTATTGCTGGGGTCGTTTCACCTTACCACCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9467.74 Da        Isoelectric Point: 4.3775

>AT50004 WP_023298920.1 NZ_CP009854:c4589187-4588945 [Enterobacter cloacae]
MSLLADLTMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIELYLDQQNSSVIREALGLWGNQQEDGLEYERKLREEW

Download         Length: 243 bp

>AT50004 NZ_CP009854:c4589187-4588945 [Enterobacter cloacae]
ATGAGTCTTTTGGCGGACCTTACTATGGGCAGAATACTGATCGATTTGTCTGATGACGTGATTCAACGACTGGATAACCT
GAAGCAGTTGCGCAACCGGCCGCGCGCCGAACTGCTGCGGGAAGCCATAGAACTGTATCTCGACCAGCAGAATTCATCGG
TTATCCGCGAAGCGCTTGGCCTGTGGGGAAATCAGCAGGAAGATGGGCTTGAGTACGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References