Detailed information of TA system TA050003

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3748217..3748874
• Replicon: chromosome
• Accession: NZ_CP009854
• Organism: UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH5

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: ECNIH5_RS17675
• Protein ID: WP_038416677.1
• Coordinates: 3748217..3748627 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: G8LDB7
• Locus tag: ECNIH5_RS17680
• Protein ID: WP_003863437.1
• Coordinates: 3748608..3748874 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECNIH5_RS17655 (ECNIH5_17655)	3744215..3745948	-	1734	WP_038416675.1	single-stranded-DNA-specific exonuclease RecJ	-
ECNIH5_RS17660 (ECNIH5_17660)	3745954..3746667	-	714	WP_022649304.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
ECNIH5_RS17665 (ECNIH5_17665)	3746696..3747592	-	897	WP_003863442.1	site-specific tyrosine recombinase XerD	-
ECNIH5_RS17670 (ECNIH5_17670)	3747694..3748215	+	522	WP_023306886.1	flavodoxin FldB	-
ECNIH5_RS17675 (ECNIH5_17675)	3748217..3748627	-	411	WP_038416677.1	protein YgfX	Toxin
ECNIH5_RS17680 (ECNIH5_17680)	3748608..3748874	-	267	WP_003863437.1	FAD assembly factor SdhE	Antitoxin
ECNIH5_RS17685 (ECNIH5_17685)	3749169..3750149	+	981	WP_038416679.1	tRNA-modifying protein YgfZ	-
ECNIH5_RS17690 (ECNIH5_17690)	3750235..3750894	-	660	WP_022649307.1	hemolysin III family protein	-
ECNIH5_RS17700 (ECNIH5_17700)	3751161..3751892	+	732	WP_022649308.1	MurR/RpiR family transcriptional regulator	-
ECNIH5_RS17705 (ECNIH5_17705)	3752009..3753442	+	1434	WP_038416681.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16337.20 Da
• Isoelectric Point: 11.4775

>T50003 WP_038416677.1 NZ_CP009854:c3748627-3748217 [Enterobacter cloacae]
VVLWQSDLRVSWRSQWMSLLLHGLVAAMVLLVPWPLSYTPLWLLLLSFVVFDSVRSQRRINSRQGEIKLLMDSRLRWQGK
EWEMIGTPWMLNTGMMLRLRRVEDNRRQHLWLAADSMDPAEWRDLRRLIVQQPTQE

Nucleotide

• Length: 411 bp

>T50003 NZ_CP009854:c3748627-3748217 [Enterobacter cloacae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACTCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCACCTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10526.96 Da
• Isoelectric Point: 4.8310

>AT50003 WP_003863437.1 NZ_CP009854:c3748874-3748608 [Enterobacter cloacae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
QERGPVAI

Nucleotide

• Length: 267 bp

>AT50003 NZ_CP009854:c3748874-3748608 [Enterobacter cloacae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCCGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A837F8P5