Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2825041..2825596 Replicon chromosome
Accession NZ_CP009854
Organism UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH5

Toxin (Protein)


Gene name shpA Uniprot ID A0A839BSP9
Locus tag ECNIH5_RS24170 Protein ID WP_071881499.1
Coordinates 2825330..2825596 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A839BUZ1
Locus tag ECNIH5_RS13320 Protein ID WP_038416295.1
Coordinates 2825041..2825343 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH5_RS13300 (ECNIH5_13300) 2820254..2821330 + 1077 WP_000227970.1 IS110 family transposase -
ECNIH5_RS24970 2821347..2821656 + 310 Protein_2619 integrase core domain-containing protein -
ECNIH5_RS13305 (ECNIH5_13305) 2821666..2822370 + 705 Protein_2620 IS5-like element IS5 family transposase -
ECNIH5_RS13310 (ECNIH5_13310) 2822669..2823580 + 912 WP_038416293.1 acetamidase/formamidase family protein -
ECNIH5_RS13315 (ECNIH5_13315) 2823577..2824920 + 1344 WP_038416294.1 APC family permease -
ECNIH5_RS13320 (ECNIH5_13320) 2825041..2825343 - 303 WP_038416295.1 BrnA antitoxin family protein Antitoxin
ECNIH5_RS24170 2825330..2825596 - 267 WP_071881499.1 BrnT family toxin Toxin
ECNIH5_RS13330 (ECNIH5_13330) 2825641..2825952 + 312 Protein_2625 IS5/IS1182 family transposase -
ECNIH5_RS25565 2825925..2825999 - 75 Protein_2626 hypothetical protein -
ECNIH5_RS13335 (ECNIH5_13335) 2826086..2826841 - 756 WP_001290572.1 SprT family zinc-dependent metalloprotease -
ECNIH5_RS13340 (ECNIH5_13340) 2826838..2830122 - 3285 WP_038416297.1 type I restriction endonuclease subunit R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2814321..2843400 29079
- inside IScluster/Tn - - 2817890..2826039 8149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(9-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10474.91 Da        Isoelectric Point: 10.0832

>T50001 WP_071881499.1 NZ_CP009854:c2825596-2825330 [Enterobacter cloacae]
IRDLFAPSLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLSLVHRIIVIMVAHSVRFESGTEVIRIISARKADSK
ERNRYEHG

Download         Length: 267 bp

>T50001 NZ_CP009854:c2825596-2825330 [Enterobacter cloacae]
ATCAGGGACTTGTTCGCACCTTCCCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGTGCTCGTCTTTGACGATCCCCG
GCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCAGCCTCGTTCATCGGATCATTGTTA
TCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGCGCCCGGAAGGCGGACAGCAAA
GAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11637.21 Da        Isoelectric Point: 9.4429

>AT50001 WP_038416295.1 NZ_CP009854:c2825343-2825041 [Enterobacter cloacae]
MNMVKHKRDNPDTLTTKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT50001 NZ_CP009854:c2825343-2825041 [Enterobacter cloacae]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGACTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BSP9

Antitoxin

Source ID Structure
AlphaFold DB A0A839BUZ1

References