Detailed information of TA system TA049992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2342908..2343647
• Replicon: chromosome
• Accession: NZ_CP009854
• Organism: UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH5

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: ECNIH5_RS11125
• Protein ID: WP_038416064.1
• Coordinates: 2343162..2343647 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: ECNIH5_RS11120
• Protein ID: WP_003857131.1
• Coordinates: 2342908..2343174 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECNIH5_RS11095 (ECNIH5_11095)	2338002..2338580	+	579	WP_022648146.1	fimbrial protein	-
ECNIH5_RS11100 (ECNIH5_11100)	2338604..2339740	+	1137	WP_038416067.1	type 1 fimbrial protein	-
ECNIH5_RS11105 (ECNIH5_11105)	2339861..2340418	+	558	WP_023300131.1	OmpH family outer membrane protein	-
ECNIH5_RS11110 (ECNIH5_11110)	2340397..2341785	-	1389	WP_038416066.1	MFS transporter	-
ECNIH5_RS11115 (ECNIH5_11115)	2341915..2342844	+	930	WP_038416065.1	LysR family transcriptional regulator	-
ECNIH5_RS11120 (ECNIH5_11120)	2342908..2343174	+	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
ECNIH5_RS11125 (ECNIH5_11125)	2343162..2343647	+	486	WP_038416064.1	GNAT family N-acetyltransferase	Toxin
ECNIH5_RS11130 (ECNIH5_11130)	2344091..2345383	-	1293	WP_038416063.1	glycosyl hydrolase family 28 protein	-
ECNIH5_RS11135 (ECNIH5_11135)	2345488..2346246	-	759	WP_022648129.1	DeoR/GlpR family DNA-binding transcription regulator	-
ECNIH5_RS11140 (ECNIH5_11140)	2346333..2346917	+	585	WP_022648128.1	NUDIX hydrolase	-
ECNIH5_RS11145 (ECNIH5_11145)	2347002..2347760	+	759	WP_032624253.1	trans-aconitate 2-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17546.28 Da
• Isoelectric Point: 9.9308

>T49992 WP_038416064.1 NZ_CP009854:2343162-2343647 [Enterobacter cloacae]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALAAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGKGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T49992 NZ_CP009854:2343162-2343647 [Enterobacter cloacae]
GTGGGACGTGTAACCGCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTACTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGCCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAAAGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT49992 WP_003857131.1 NZ_CP009854:2342908-2343174 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT49992 NZ_CP009854:2342908-2343174 [Enterobacter cloacae]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9