Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1095023..1095644 Replicon chromosome
Accession NZ_CP009854
Organism UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH5

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag ECNIH5_RS05180 Protein ID WP_015571250.1
Coordinates 1095023..1095241 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag ECNIH5_RS05185 Protein ID WP_006809850.1
Coordinates 1095270..1095644 (-) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH5_RS05150 (ECNIH5_05150) 1091035..1091295 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
ECNIH5_RS05155 (ECNIH5_05155) 1091298..1091438 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
ECNIH5_RS05160 (ECNIH5_05160) 1091435..1092145 - 711 WP_038415530.1 GNAT family protein -
ECNIH5_RS05165 (ECNIH5_05165) 1092248..1093708 + 1461 WP_038415532.1 PLP-dependent aminotransferase family protein -
ECNIH5_RS05170 (ECNIH5_05170) 1093680..1094147 - 468 WP_022647269.1 YlaC family protein -
ECNIH5_RS05175 (ECNIH5_05175) 1094264..1094815 - 552 WP_022647270.1 maltose O-acetyltransferase -
ECNIH5_RS05180 (ECNIH5_05180) 1095023..1095241 - 219 WP_015571250.1 HHA domain-containing protein Toxin
ECNIH5_RS05185 (ECNIH5_05185) 1095270..1095644 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
ECNIH5_RS05190 (ECNIH5_05190) 1096154..1099300 - 3147 WP_023299464.1 multidrug efflux RND transporter permease subunit AcrB -
ECNIH5_RS05195 (ECNIH5_05195) 1099323..1100516 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(13-69)

Antitoxin

(1-114)


Sequences


Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T49991 WP_015571250.1 NZ_CP009854:c1095241-1095023 [Enterobacter cloacae]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T49991 NZ_CP009854:c1095241-1095023 [Enterobacter cloacae]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGTGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT49991 WP_006809850.1 NZ_CP009854:c1095644-1095270 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT49991 NZ_CP009854:c1095644-1095270 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A837FFM2


Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References