Detailed information of TA system TA049973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 1928512..1929028
• Replicon: chromosome
• Accession: NZ_CP009847
• Organism: Vibrio parahaemolyticus strain FORC_004

Toxin (Protein)

• Gene name: relB
• Uniprot ID: U2ZZV8
• Locus tag: FORC4_RS08695
• Protein ID: WP_005464459.1
• Coordinates: 1928759..1929028 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: FORC4_RS08690
• Protein ID: WP_025548943.1
• Coordinates: 1928512..1928766 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC4_RS08660	1924579..1925040	-	462	WP_057619416.1	hypothetical protein	-
FORC4_RS08665	1925382..1925828	+	447	WP_005500348.1	DUF3291 domain-containing protein	-
FORC4_RS08670	1925943..1926146	+	204	WP_025545837.1	lactoylglutathione lyase	-
FORC4_RS25445	1926230..1926343	-	114	Protein_1691	IS481 family transposase	-
FORC4_RS24005	1927579..1927734	+	156	Protein_1693	SDR family oxidoreductase	-
FORC4_RS08685	1928022..1928288	+	267	WP_005464625.1	hypothetical protein	-
FORC4_RS08690	1928512..1928766	+	255	WP_025548943.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FORC4_RS08695	1928759..1929028	+	270	WP_005464459.1	Txe/YoeB family addiction module toxin	Toxin
FORC4_RS08700	1929245..1929466	+	222	Protein_1697	transposase	-
FORC4_RS25450	1929550..1930350	+	801	WP_086482363.1	IS3 family transposase	-
FORC4_RS25455	1930719..1930774	+	56	Protein_1699	DUF3265 domain-containing protein	-
FORC4_RS08710	1930961..1931851	+	891	WP_005485669.1	hypothetical protein	-
FORC4_RS08715	1931932..1932342	+	411	WP_024701711.1	hypothetical protein	-
FORC4_RS08720	1932521..1932954	+	434	Protein_1702	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1919986..1954361	34375
	-	inside	Integron	-	-	1927738..1932487	4749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10614.96 Da
• Isoelectric Point: 7.9816

>T49973 WP_005464459.1 NZ_CP009847:1928759-1929028 [Vibrio parahaemolyticus]
MSSSQRLLSWTDDAWDDYLYWQTQDKKTLKRINKLINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Nucleotide

• Length: 270 bp

>T49973 NZ_CP009847:1928759-1929028 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTGTTATCGTGGACTGATGATGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAATAAACTCATCAATGATGTTAAGCGCTCTCCATTTGAGGGCATTGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATTTCATGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9511.68 Da
• Isoelectric Point: 5.0623

>AT49973 WP_025548943.1 NZ_CP009847:1928512-1928766 [Vibrio parahaemolyticus]
MRIVSFTEARNSLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNVEHLNRSIAQYRAGKTTARE
LIDE

Nucleotide

• Length: 255 bp

>AT49973 NZ_CP009847:1928512-1928766 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATAGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACTGTTCATT
TACTACGCTCTCCTCAAAACGTTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG

Structures

Toxin

• AlphaFold DB: U2ZZV8