Detailed information of TA system TA049959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 40984..41518
• Replicon: plasmid pCD
• Accession: NZ_CP009842
• Organism: Yersinia pestis strain Dodson

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: CH61_RS00270
• Protein ID: WP_002213360.1
• Coordinates: 41219..41518 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: CH61_RS00265
• Protein ID: WP_002224342.1
• Coordinates: 40984..41226 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH61_RS00250	37594..38760	+	1167	WP_002220902.1	plasmid-partitioning protein SopA	-
CH61_RS00255	38757..39722	+	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
CH61_RS24970	39882..40118	+	237	WP_154020274.1	hypothetical protein	-
CH61_RS25135	40267..40440	-	174	WP_002213357.1	hypothetical protein	-
CH61_RS24005	40526..40818	+	293	Protein_54	IS6 family transposase	-
CH61_RS00265	40984..41226	+	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CH61_RS00270	41219..41518	+	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH61_RS00275	41658..42317	-	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
CH61_RS00280	42511..42903	+	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
CH61_RS00285	42966..43610	-	645	Protein_59	IS3 family transposase	-
CH61_RS24410	43713..43961	+	249	Protein_60	IS21 family transposase	-
CH61_RS00300	44028..44810	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
CH61_RS00305	44807..45829	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscP / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV / sycD/lcrH / yopB / yopD / yopM / sycT / yopT / yopK/yopQ / yopE / sycE/yerA / sycH / yadA / yadA / yopO/ypkA / yopJ/yopP / yopH	1..70305	70305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T49959 WP_002213360.1 NZ_CP009842:41219-41518 [Yersinia pestis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T49959 NZ_CP009842:41219-41518 [Yersinia pestis]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT49959 WP_002224342.1 NZ_CP009842:40984-41226 [Yersinia pestis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT49959 NZ_CP009842:40984-41226 [Yersinia pestis]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6