Detailed information of TA system TA049903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2006516..2007045
• Replicon: chromosome
• Accession: NZ_CP009828
• Organism: Staphylococcus aureus strain MS4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: NI36_RS10080
• Protein ID: WP_000621175.1
• Coordinates: 2006516..2006878 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: NI36_RS10085
• Protein ID: WP_000948331.1
• Coordinates: 2006875..2007045 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NI36_RS10055	2003494..2004264	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
NI36_RS10060	2004239..2004718	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
NI36_RS10065	2004720..2005046	-	327	WP_001052491.1	anti-sigma factor antagonist	-
NI36_RS10070	2005165..2006166	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
NI36_RS10080	2006516..2006878	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NI36_RS10085	2006875..2007045	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
NI36_RS10090	2007130..2008278	-	1149	WP_001280702.1	alanine racemase	-
NI36_RS10095	2008344..2008703	-	360	WP_000581194.1	holo-ACP synthase	-
NI36_RS10100	2008707..2009198	-	492	WP_001205915.1	PH domain-containing protein	-
NI36_RS10105	2009185..2010768	-	1584	WP_001294642.1	PH domain-containing protein	-
NI36_RS10110	2010761..2011240	-	480	WP_001287083.1	hypothetical protein	-
NI36_RS10115	2011449..2012009	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T49903 WP_000621175.1 NZ_CP009828:c2006878-2006516 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T49903 NZ_CP009828:c2006878-2006516 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT49903 WP_000948331.1 NZ_CP009828:c2007045-2006875 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT49903 NZ_CP009828:c2007045-2006875 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1