Detailed information of TA system TA049897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 35880..36550
• Replicon: plasmid pXF39
• Accession: NZ_CP009827
• Organism: Xylella fastidiosa strain Pr8x

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: XFPR_RS12230
• Protein ID: WP_031337234.1
• Coordinates: 35880..36230 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: XFPR_RS12235
• Protein ID: WP_023908245.1
• Coordinates: 36227..36550 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFPR_RS12200	31366..31614	-	249	WP_196432213.1	transcriptional regulator	-
XFPR_RS12205	31623..32162	-	540	WP_075584708.1	hypothetical protein	-
XFPR_RS12210	32137..33342	-	1206	WP_023908241.1	ParB/RepB/Spo0J family partition protein	-
XFPR_RS12215	33339..34103	-	765	WP_010895252.1	ParA family protein	-
XFPR_RS12220	34264..34674	-	411	WP_023908242.1	single-stranded DNA-binding protein	-
XFPR_RS12225	34748..35539	-	792	WP_031337233.1	DUF3560 domain-containing protein	-
XFPR_RS12230	35880..36230	+	351	WP_031337234.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XFPR_RS12235	36227..36550	+	324	WP_023908245.1	helix-turn-helix domain-containing protein	Antitoxin
XFPR_RS12240	36558..36965	-	408	WP_023908246.1	hypothetical protein	-
XFPR_RS12245	37028..37363	-	336	WP_023908247.1	helix-turn-helix domain-containing protein	-
XFPR_RS12250	37344..37721	-	378	WP_023908248.1	type II toxin-antitoxin system RelE/ParE family toxin	-
XFPR_RS12255	38557..39333	-	777	WP_023908250.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..39580	39580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-82)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12740.62 Da
• Isoelectric Point: 8.0810

>T49897 WP_031337234.1 NZ_CP009827:35880-36230 [Xylella fastidiosa]
MKPLVWIASAKKDLMAMPDDVQDTFGYALHLAQTGEKHDQAKPLKGFGSASVLEVVEDSDGGTYRGVYTVRFGNAVYVLH
CFQKKSTHGIATPKPDIDLIRDRLKVAEAHAKGELK

Nucleotide

• Length: 351 bp

>T49897 NZ_CP009827:35880-36230 [Xylella fastidiosa]
ATGAAACCGCTAGTTTGGATCGCTTCGGCCAAGAAAGACCTGATGGCGATGCCGGACGACGTACAGGACACTTTCGGGTA
CGCGCTGCATTTGGCTCAGACCGGCGAGAAGCACGACCAGGCCAAGCCGCTCAAAGGATTCGGTTCGGCTAGCGTGCTCG
AAGTGGTCGAGGATTCAGACGGCGGCACATACCGCGGGGTCTACACGGTGCGATTCGGAAACGCAGTGTATGTCCTGCAT
TGCTTCCAGAAGAAATCGACGCATGGCATAGCGACACCAAAGCCGGATATCGACCTCATTCGAGATCGATTGAAGGTCGC
CGAGGCGCACGCGAAAGGAGAACTGAAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11595.43 Da
• Isoelectric Point: 5.1186

>AT49897 WP_023908245.1 NZ_CP009827:36227-36550 [Xylella fastidiosa]
MIEIEEGSGNVYADLGIPDADEMLVKAQLATKIGEIIKGRGWTQQEAANVLGMTQPKLSKMLRGQFRGISEAKMLEYLAR
LGQQVQIVIGPARLTNDAGHVEVVFAA

Nucleotide

• Length: 324 bp

>AT49897 NZ_CP009827:36227-36550 [Xylella fastidiosa]
ATGATCGAAATTGAAGAAGGTAGCGGCAACGTCTATGCCGATCTTGGTATTCCTGACGCCGACGAAATGCTGGTGAAGGC
GCAGCTTGCTACGAAGATCGGCGAGATCATCAAGGGTCGCGGATGGACTCAGCAAGAAGCGGCCAACGTGCTCGGCATGA
CACAACCCAAGCTGTCGAAGATGCTGCGCGGACAGTTCCGAGGTATCAGCGAAGCGAAGATGCTGGAATACCTCGCGCGG
CTCGGTCAGCAAGTTCAGATCGTCATCGGTCCCGCTCGCCTTACAAACGACGCGGGTCACGTCGAAGTCGTATTCGCGGC
CTAA