Detailed information of TA system TA049886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 21596..22182
• Replicon: plasmid pXF27-J1
• Accession: NZ_CP009824
• Organism: Xylella fastidiosa strain J1a12

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: OY18_RS13830
• Protein ID: WP_060870251.1
• Coordinates: 21596..21922 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5LL99
• Locus tag: OY18_RS13835
• Protein ID: WP_013087943.1
• Coordinates: 21919..22182 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OY18_RS13805 (OY18_13005)	17417..17752	+	336	WP_060871680.1	FxLYD domain-containing protein	-
OY18_RS13810 (OY18_13010)	17812..18444	-	633	WP_229926363.1	hypothetical protein	-
OY18_RS14790	18398..18613	-	216	WP_173406029.1	hypothetical protein	-
OY18_RS13815 (OY18_13015)	18653..19789	+	1137	WP_229926364.1	relaxase/mobilization nuclease domain-containing protein	-
OY18_RS13820 (OY18_13020)	19880..21037	-	1158	WP_060871686.1	RNA-guided endonuclease TnpB family protein	-
OY18_RS13825 (OY18_13025)	21060..21476	+	417	WP_060871682.1	IS200/IS605 family transposase	-
OY18_RS13830 (OY18_13030)	21596..21922	-	327	WP_060870251.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OY18_RS13835 (OY18_13035)	21919..22182	-	264	WP_013087943.1	antitoxin	Antitoxin
OY18_RS13840 (OY18_13040)	22239..23339	-	1101	WP_060870252.1	zeta toxin family protein	-
OY18_RS13845 (OY18_13045)	23345..23599	-	255	WP_060870253.1	hypothetical protein	-
OY18_RS13850 (OY18_13050)	23618..24196	-	579	WP_229926365.1	plasmid mobilization relaxosome protein MobC	-
OY18_RS13855 (OY18_13055)	24608..24868	+	261	WP_229926366.1	hypothetical protein	-
OY18_RS13860 (OY18_13060)	24881..25633	+	753	WP_013087971.1	hypothetical protein	-
OY18_RS13865 (OY18_13065)	25634..26077	+	444	WP_060870257.1	hypothetical protein	-
OY18_RS13870 (OY18_13070)	26182..26508	-	327	WP_060870258.1	type II toxin-antitoxin system PemK/MazF family toxin	-
OY18_RS13875	26505..26729	-	225	WP_060870259.1	antitoxin MazE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..27268	27268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-102)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11809.78 Da
• Isoelectric Point: 9.3233

>T49886 WP_060870251.1 NZ_CP009824:c21922-21596 [Xylella fastidiosa]
MKRGDVYMVDLEPTAGHEQRGHRPVVVISSERFNRLTSCPVILPITNGGEFASRLGFAVELLGTITTGVVRCDQPRALDL
LARKARKVESLPPNILADVLAKAVTIFQ

Nucleotide

• Length: 327 bp

>T49886 NZ_CP009824:c21922-21596 [Xylella fastidiosa]
ATGAAACGCGGCGATGTTTACATGGTCGATCTGGAGCCAACCGCCGGGCACGAACAACGCGGTCACCGTCCGGTGGTTGT
GATCTCCAGTGAGCGATTCAATCGGCTTACCTCTTGTCCGGTGATCCTTCCAATCACAAACGGCGGCGAGTTCGCCTCCC
GCCTCGGGTTCGCTGTGGAATTACTAGGTACGATTACAACGGGCGTCGTTCGATGTGATCAACCACGTGCGCTCGACTTA
CTCGCTCGCAAAGCCCGCAAAGTGGAAAGCCTACCACCAAACATCTTGGCCGATGTCTTGGCAAAAGCTGTCACCATCTT
CCAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9365.77 Da
• Isoelectric Point: 4.3588

>AT49886 WP_013087943.1 NZ_CP009824:c22182-21919 [Xylella fastidiosa]
MHTTNLRKVGGSIMLAVPPAFLDQLHLEVGATVGLAVTDGRLVIEPVLHPQYTLDQLLAEAEASGAYPLPPEEREWVDAP
AVGRELI

Nucleotide

• Length: 264 bp

>AT49886 NZ_CP009824:c22182-21919 [Xylella fastidiosa]
ATGCACACCACAAACCTTCGTAAAGTCGGCGGATCGATCATGCTGGCCGTTCCCCCCGCTTTCCTTGATCAGCTTCATCT
TGAGGTCGGGGCAACGGTCGGCTTAGCCGTCACAGACGGTCGTCTGGTCATTGAGCCAGTCCTACATCCTCAGTACACGC
TGGATCAACTGCTGGCCGAAGCGGAAGCCTCCGGGGCTTACCCGTTGCCGCCCGAGGAACGTGAATGGGTCGATGCTCCA
GCAGTAGGACGCGAACTGATATGA

Structures

Antitoxin

• AlphaFold DB: A0A060H856