Detailed information of TA system TA049885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2656..3139
• Replicon: plasmid pXF27-J1
• Accession: NZ_CP009824
• Organism: Xylella fastidiosa strain J1a12

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: OY18_RS13720
• Protein ID: WP_060871666.1
• Coordinates: 2870..3139 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: OY18_RS13715
• Protein ID: WP_060871665.1
• Coordinates: 2656..2886 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OY18_RS13695 (OY18_12900)	18..1121	-	1104	WP_081089762.1	replication protein RepA	-
OY18_RS13700 (OY18_12905)	1148..1751	-	604	Protein_1	recombinase family protein	-
OY18_RS13705 (OY18_12910)	1973..2203	+	231	WP_042464041.1	hypothetical protein	-
OY18_RS13710 (OY18_12915)	2190..2516	+	327	WP_060872373.1	type II toxin-antitoxin system PemK/MazF family toxin	-
OY18_RS13715 (OY18_12920)	2656..2886	+	231	WP_060871665.1	DUF6290 family protein	Antitoxin
OY18_RS13720 (OY18_12925)	2870..3139	+	270	WP_060871666.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OY18_RS13725 (OY18_12930)	3188..3469	-	282	WP_229926367.1	hypothetical protein	-
OY18_RS15320	3438..3602	-	165	WP_229926368.1	hypothetical protein	-
OY18_RS13735 (OY18_12940)	4453..4632	+	180	WP_081089764.1	hypothetical protein	-
OY18_RS13740 (OY18_12945)	4720..5115	+	396	WP_229926369.1	hypothetical protein	-
OY18_RS13745 (OY18_12950)	5118..5438	+	321	WP_060871671.1	TrbC/VirB2 family protein	-
OY18_RS13750 (OY18_12955)	5443..5826	+	384	WP_013087959.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..27268	27268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10295.89 Da
• Isoelectric Point: 10.2721

>T49885 WP_060871666.1 NZ_CP009824:2870-3139 [Xylella fastidiosa]
MAWKVELSSLAQKNLDQLDPQIARRVLSFLHERVAPLDDPRSIGESLKGSRLGEFWKYRVGDYRIISSIEDGALRILVVK
IGNRREVYR

Nucleotide

• Length: 270 bp

>T49885 NZ_CP009824:2870-3139 [Xylella fastidiosa]
ATGGCATGGAAGGTTGAGCTTTCGTCGTTGGCTCAAAAAAACCTTGACCAGCTTGACCCACAAATTGCACGGCGTGTCTT
GTCCTTTCTGCATGAGCGCGTGGCCCCGCTGGACGATCCGCGCAGCATCGGTGAATCCCTCAAGGGTTCGCGCCTTGGTG
AGTTTTGGAAGTACCGCGTGGGTGATTACCGGATTATCAGCAGCATCGAGGACGGCGCTTTGCGCATCCTCGTTGTGAAG
ATCGGCAACCGCCGCGAGGTGTACCGCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8675.92 Da
• Isoelectric Point: 4.3690

>AT49885 WP_060871665.1 NZ_CP009824:2656-2886 [Xylella fastidiosa]
MVSVSLRLPDEVTQRLENLAALTGRSKTFYMVEAIREHIDDLEDLYLAECELEAIRSGKSQTVPLEEVMKRYGMEG

Nucleotide

• Length: 231 bp

>AT49885 NZ_CP009824:2656-2886 [Xylella fastidiosa]
ATGGTTTCCGTATCACTGCGCCTGCCTGATGAAGTCACACAACGACTTGAGAACTTGGCCGCGCTCACAGGACGTAGCAA
GACCTTCTACATGGTGGAAGCCATCCGCGAACACATCGACGACCTGGAAGACTTGTATTTGGCCGAGTGTGAGCTTGAAG
CGATCCGTTCTGGCAAGTCGCAAACCGTACCACTCGAAGAAGTGATGAAACGCTATGGCATGGAAGGTTGA