Detailed information of TA system TA049879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1739926..1740518
• Replicon: chromosome
• Accession: NZ_CP009823
• Organism: Xylella fastidiosa strain J1a12

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q9PD07
• Locus tag: OY18_RS08415
• Protein ID: WP_010894094.1
• Coordinates: 1740222..1740518 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: OY18_RS08410
• Protein ID: WP_004085592.1
• Coordinates: 1739926..1740219 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OY18_RS08380 (OY18_07935)	1736301..1736618	+	318	WP_229926248.1	Blp family class II bacteriocin	-
OY18_RS08385 (OY18_07940)	1736968..1737738	+	771	WP_088569704.1	hypothetical protein	-
OY18_RS08390 (OY18_07945)	1737738..1738055	+	318	WP_069107095.1	hypothetical protein	-
OY18_RS08395 (OY18_07950)	1738052..1738882	+	831	WP_060872216.1	hypothetical protein	-
OY18_RS08400 (OY18_07955)	1738879..1739520	+	642	WP_060872217.1	Gp138 family membrane-puncturing spike protein	-
OY18_RS08405 (OY18_07960)	1739532..1739870	+	339	WP_229926249.1	hypothetical protein	-
OY18_RS08410 (OY18_07965)	1739926..1740219	-	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
OY18_RS08415 (OY18_07970)	1740222..1740518	-	297	WP_010894094.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OY18_RS15045	1740621..1740803	+	183	WP_010894095.1	baseplate J/gp47 family protein	-
OY18_RS15050	1740866..1741015	+	150	WP_229926250.1	hypothetical protein	-
OY18_RS08425 (OY18_07980)	1741119..1741565	-	447	WP_031336674.1	hypothetical protein	-
OY18_RS08430 (OY18_07985)	1741807..1742784	-	978	WP_023906642.1	hypothetical protein	-
OY18_RS08435 (OY18_07990)	1742781..1743656	-	876	WP_023906641.1	ABC transporter ATP-binding protein	-
OY18_RS14705	1743835..1743996	-	162	WP_155641627.1	hypothetical protein	-
OY18_RS08440 (OY18_07995)	1744402..1744980	+	579	WP_046419686.1	glutathione peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1714961..1740518	25557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11167.73 Da
• Isoelectric Point: 9.8173

>T49879 WP_010894094.1 NZ_CP009823:c1740518-1740222 [Xylella fastidiosa]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQQRGDTIYLLLCGGDKG
LQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T49879 NZ_CP009823:c1740518-1740222 [Xylella fastidiosa]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCACGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCAGCGTGGCGACACGATCTACTTGCTACTTTGCGGCGGTGACAAAGGA
TTACAAGCGCGTGACATCAAGACTGCGCTGCACCTCTCTGAACAATGGAGCGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT49879 WP_004085592.1 NZ_CP009823:c1740219-1739926 [Xylella fastidiosa]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT49879 NZ_CP009823:c1740219-1739926 [Xylella fastidiosa]
ATGACCGAGAAACTGACGAGCTACGATCCGGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCCGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTAGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACGACATTAGCC
GTGATGAAGGCGCTAGGGATCGAGCTCTCTGCGAAACCGTCTGGTGTTCACTGA

Structures

Toxin

• AlphaFold DB: A0A1S0V711