Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3252977..3253596 Replicon chromosome
Accession NZ_CP009801
Organism Yersinia intermedia strain Y228

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag CH53_RS14230 Protein ID WP_002208622.1
Coordinates 3252977..3253180 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0T9M685
Locus tag CH53_RS14235 Protein ID WP_005182270.1
Coordinates 3253228..3253596 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH53_RS14205 3248232..3248570 + 339 WP_002208627.1 P-II family nitrogen regulator -
CH53_RS14210 3248610..3249899 + 1290 WP_032905738.1 ammonium transporter AmtB -
CH53_RS14215 3249979..3250839 - 861 WP_042569284.1 acyl-CoA thioesterase II -
CH53_RS14220 3251098..3251652 + 555 WP_005182263.1 YbaY family lipoprotein -
CH53_RS14225 3251743..3252048 - 306 WP_186004081.1 MGMT family protein -
CH53_RS14230 3252977..3253180 - 204 WP_002208622.1 expression modulating protein YmoA Toxin
CH53_RS14235 3253228..3253596 - 369 WP_005182270.1 Hha toxicity modulator TomB Antitoxin
CH53_RS14240 3253755..3254108 - 354 WP_032905603.1 hypothetical protein -
CH53_RS14245 3254884..3255621 + 738 WP_050073470.1 ABC transporter ATP-binding protein -
CH53_RS14250 3255618..3256475 + 858 WP_042569286.1 metal ABC transporter permease -
CH53_RS14255 3256504..3257382 + 879 WP_005182278.1 metal ABC transporter substrate-binding protein -
CH53_RS14260 3257549..3257692 - 144 WP_002208618.1 type B 50S ribosomal protein L36 -
CH53_RS14265 3257695..3257967 - 273 WP_032905607.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T49860 WP_002208622.1 NZ_CP009801:c3253180-3252977 [Yersinia intermedia]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T49860 NZ_CP009801:c3253180-3252977 [Yersinia intermedia]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATTGATACATTAGAACGTGTAATTGAAAAGAATAA
GTACGAGCTTTCCGATGATGAGCTGGAATTGTTTTACTCAGCAGCAGACCATCGCCTGGCTGAACTCACAATGAATAAGC
TTTACGATAAAATTCCTCCTACGGTTTGGCAACATGTGAAATAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14222.93 Da        Isoelectric Point: 4.4314

>AT49860 WP_005182270.1 NZ_CP009801:c3253596-3253228 [Yersinia intermedia]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDDGDLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKERLFRLFSGEYTCTLMKT

Download         Length: 369 bp

>AT49860 NZ_CP009801:c3253596-3253228 [Yersinia intermedia]
ATGGATGAGTACTCGCCTAAACGGCATGACATTGCCCAGTTGAAGTTTCTATGTGAGAGCCTGTATGATGAAGGCATTGC
TACCCTTGGTGACAGCCATCATGGCTGGGTAAATGACCCAACATCTGCCGTAAATCTGCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGACGGCGACCTTTCAGAGTTAGTGGAAGAATATTTA
GACGATACATATACTCTGTTTAGTAGTTACGGAATCAATGATCCTGAATTGAGGCGCTGGCAAAAAACCAAAGAGCGCTT
ATTCAGACTTTTCTCAGGGGAATACACCTGTACCCTGATGAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2K5S

Antitoxin

Source ID Structure
AlphaFold DB A0A0T9M685

References