Detailed information of TA system TA049856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2448348..2449977
• Replicon: chromosome
• Accession: NZ_CP009801
• Organism: Yersinia intermedia strain Y228

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: CH53_RS10700
• Protein ID: WP_042568982.1
• Coordinates: 2448348..2449676 (-)
• Length: 443 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0T9MED0
• Locus tag: CH53_RS10705
• Protein ID: WP_005191449.1
• Coordinates: 2449678..2449977 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH53_RS10685	2443773..2444126	+	354	WP_032907677.1	MmcQ/YjbR family DNA-binding protein	-
CH53_RS10690	2444265..2447096	-	2832	WP_005191457.1	excinuclease ABC subunit UvrA	-
CH53_RS10695	2447497..2448045	+	549	WP_005191456.1	single-stranded DNA-binding protein SSB1	-
CH53_RS10700	2448348..2449676	-	1329	WP_042568982.1	type II toxin-antitoxin system HipA family toxin	Toxin
CH53_RS10705	2449678..2449977	-	300	WP_005191449.1	helix-turn-helix transcriptional regulator	Antitoxin
CH53_RS10710	2450247..2450915	+	669	WP_005191448.1	glutathione S-transferase	-
CH53_RS10715	2451286..2452653	+	1368	WP_005191447.1	NCS2 family permease	-
CH53_RS10720	2452872..2454521	+	1650	WP_005191446.1	Na+/H+ antiporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 443 a.a.
• Molecular weight: 50164.05 Da
• Isoelectric Point: 6.8620

>T49856 WP_042568982.1 NZ_CP009801:c2449676-2448348 [Yersinia intermedia]
MTRRIQRLNIWMNGQYVGYWEKTRGEESLVYAQEWIENAQGRPLSLSLPFTANNQVYRGNIVRDYFDNLLPDSDDIRRRL
ATRYHADSINPFDLLFELGRDCVGAIQLLANDTPPADLFSIQQQPLTEHDVARILRNSWSDNTFVHPEHNEDLRLSIAGA
QEKTALLFHDDRWCLPLGSTPTTHIFKLPLGLVGNMQADMRTSVENEWLCAKILASYNLPVARSEIGQFEDQKALIVERF
DRRLSRDNRWIVRLPQEDMCQASGTSPLKKYQMDGGLGITTIMGILSGSDNASEDRNLFFKTQIIFWLLAATDGHAKNFS
IAHLPRNSYHLTPLYNVLSTHPIIGSSHNQIAIQKAKLAMAVRGSENHYLINQIQRRHWRKQAELVGLGAYLADSIIDEV
ITATPRVIAQIQAQLPANFPTDLAESILTGLHRQCDKIAAMP

Nucleotide

• Length: 1329 bp

>T49856 NZ_CP009801:c2449676-2448348 [Yersinia intermedia]
ATGACTCGCCGCATTCAACGGCTCAATATCTGGATGAACGGTCAGTATGTTGGCTATTGGGAAAAAACCCGAGGCGAAGA
ATCACTGGTTTATGCTCAAGAATGGATAGAGAACGCGCAAGGCCGCCCACTGTCATTGTCATTACCTTTTACTGCAAATA
ATCAGGTTTATAGAGGCAATATTGTCCGGGACTATTTTGATAACTTATTACCCGATAGCGATGATATCCGGCGGCGTCTG
GCAACCAGATATCACGCTGACAGCATCAACCCTTTCGATCTACTCTTTGAATTAGGGAGAGATTGTGTCGGCGCAATACA
ATTGCTGGCAAATGATACCCCCCCTGCCGATCTATTCTCAATTCAACAACAGCCACTAACTGAGCACGATGTGGCTCGTA
TCCTACGAAACTCATGGTCTGACAATACATTTGTGCACCCTGAACACAATGAAGACCTCAGATTATCTATCGCGGGGGCA
CAAGAAAAAACAGCGCTGTTGTTTCACGATGACCGGTGGTGTCTCCCGTTGGGCAGCACTCCCACAACCCATATTTTCAA
ACTTCCTTTGGGCTTGGTCGGCAATATGCAAGCTGATATGAGGACTTCTGTTGAAAACGAATGGCTGTGTGCAAAAATTC
TGGCAAGTTATAACTTGCCAGTTGCCCGTAGTGAAATTGGCCAGTTTGAAGATCAAAAAGCCCTGATTGTTGAACGTTTT
GACCGGCGGTTATCCCGTGACAATCGTTGGATTGTCAGATTACCGCAGGAAGATATGTGTCAGGCCTCCGGGACTTCACC
CTTGAAAAAATACCAAATGGATGGCGGCCTTGGTATTACAACTATCATGGGGATTTTAAGTGGTTCGGATAATGCCAGTG
AAGACAGAAACCTCTTCTTTAAAACACAGATTATTTTCTGGTTACTGGCAGCAACCGATGGGCATGCAAAAAATTTCAGC
ATTGCTCATCTCCCCAGAAATAGTTACCACCTCACGCCACTTTATAATGTGCTATCGACCCATCCGATTATCGGCTCAAG
TCATAATCAAATTGCGATACAAAAAGCCAAGTTAGCGATGGCCGTTCGAGGGAGTGAAAATCACTATTTAATTAACCAGA
TACAGCGGCGCCATTGGCGAAAACAGGCCGAGCTTGTTGGGCTAGGAGCGTATCTTGCTGATAGTATTATTGATGAAGTT
ATTACCGCAACCCCGAGGGTAATAGCACAAATACAAGCCCAGCTTCCCGCTAATTTTCCAACTGATTTAGCAGAAAGTAT
CCTCACAGGTCTTCATCGACAGTGTGATAAAATAGCGGCAATGCCTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11268.02 Da
• Isoelectric Point: 9.4879

>AT49856 WP_005191449.1 NZ_CP009801:c2449977-2449678 [Yersinia intermedia]
MDYPIKILSQLRPTLIGFRKQKGLTQASLAQLLGITQQSYAKLEADPASASIERLFKVLQLLDVELILSEKKNVRLYSSK
THQPEESQTLNLPSKQEDW

Nucleotide

• Length: 300 bp

>AT49856 NZ_CP009801:c2449977-2449678 [Yersinia intermedia]
ATGGACTATCCCATTAAAATTTTGAGTCAATTACGCCCTACCTTGATTGGCTTTCGTAAGCAGAAAGGGCTAACTCAGGC
CAGCCTGGCACAACTGTTAGGTATCACCCAGCAATCCTATGCAAAATTGGAGGCAGATCCTGCATCGGCCAGTATTGAAC
GGCTTTTTAAAGTATTACAACTTTTAGACGTAGAACTCATCCTCAGTGAGAAAAAAAACGTCAGGCTGTATTCAAGTAAA
ACCCACCAGCCAGAAGAAAGTCAGACGCTAAATTTGCCGTCAAAACAAGAGGATTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0T9MED0