Detailed information of TA system TA049848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1092697..1093352
• Replicon: chromosome
• Accession: NZ_CP009799
• Organism: Burkholderia ambifaria AMMD

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q0B7Y2
• Locus tag: CH72_RS21150
• Protein ID: WP_011659179.1
• Coordinates: 1092697..1093116 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CH72_RS21155
• Protein ID: WP_041491669.1
• Coordinates: 1093113..1093352 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH72_RS21125	1087984..1088778	+	795	WP_011659174.1	AraC family transcriptional regulator	-
CH72_RS21130	1089138..1090097	+	960	WP_011659175.1	DMT family transporter	-
CH72_RS21135	1090223..1090795	+	573	WP_011659176.1	hypothetical protein	-
CH72_RS21140	1090895..1091029	-	135	WP_011659177.1	entericidin A/B family lipoprotein	-
CH72_RS21145	1091265..1092560	+	1296	WP_011659178.1	aspartate carbamoyltransferase	-
CH72_RS21150	1092697..1093116	-	420	WP_011659179.1	type II toxin-antitoxin system VapC family toxin	Toxin
CH72_RS21155	1093113..1093352	-	240	WP_041491669.1	Arc family DNA-binding protein	Antitoxin
CH72_RS21160	1093673..1095916	+	2244	WP_011659181.1	TonB-dependent siderophore receptor	-
CH72_RS21165	1095920..1096675	+	756	WP_011659182.1	sel1 repeat family protein	-
CH72_RS21170	1096672..1097355	+	684	WP_011659183.1	Fe2+-dependent dioxygenase	-
CH72_RS21175	1097455..1097694	+	240	WP_011659184.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CH72_RS21180	1097691..1098086	+	396	WP_011659185.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-44)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15056.31 Da
• Isoelectric Point: 7.0177

>T49848 WP_011659179.1 NZ_CP009799:c1093116-1092697 [Burkholderia ambifaria AMMD]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLHQSIEQRVVPLFRGRILPFDD
AASKAYASLRAKARATGNAMASADGFIAATAAANGLIVATRDVAPFEAAGLRVIDPWAR

Nucleotide

• Length: 420 bp

>T49848 NZ_CP009799:c1093116-1092697 [Burkholderia ambifaria AMMD]
ATGATCCTCGTCGATACCAACGTCATTTCCGAGCCGCTGCGGCGCGAGCCGAGTGCGGCCGTGATCGAATGGCTCGATGC
GCAGAATGTCGAGACGCTGTTTCTTGCCGCGATCAGTCTCGCGGAAATGCGCTTCGGCGTGGCCGCGTTGCCGGAAGGGA
GAAGGCGGGACTGGCTGCACCAAAGCATCGAGCAGCGCGTCGTGCCGCTGTTTCGCGGCCGGATCCTGCCGTTCGACGAC
GCGGCGAGTAAAGCCTATGCGAGCCTTCGTGCGAAGGCTCGTGCTACGGGCAACGCGATGGCGTCTGCTGACGGATTTAT
CGCTGCCACGGCCGCGGCGAACGGCTTGATCGTGGCGACGCGCGACGTCGCGCCATTCGAAGCGGCGGGCTTGCGCGTGA
TCGACCCGTGGGCCCGGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8618.76 Da
• Isoelectric Point: 6.4688

>AT49848 WP_041491669.1 NZ_CP009799:c1093352-1093113 [Burkholderia ambifaria AMMD]
MPVITVRNLPDEVHRALRIRAAQHGRSTEAEVRAILEEAVRPTGRAKLGSLLAEIGREAGGVDFENPRESSPTAPMSFE

Nucleotide

• Length: 240 bp

>AT49848 NZ_CP009799:c1093352-1093113 [Burkholderia ambifaria AMMD]
ATGCCGGTGATTACCGTTCGAAATTTGCCCGACGAGGTGCATCGCGCGCTTCGCATCCGTGCGGCGCAACATGGTCGGAG
TACCGAAGCGGAGGTGCGCGCCATCCTCGAGGAGGCTGTTCGGCCCACCGGCCGGGCGAAGCTGGGGTCGCTGCTGGCCG
AAATCGGGCGGGAGGCGGGCGGTGTCGATTTCGAGAATCCGCGCGAGAGCTCCCCAACCGCTCCGATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: Q0B7Y2