Detailed information of TA system TA049844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3528842..3529527
• Replicon: chromosome
• Accession: NZ_CP009798
• Organism: Burkholderia ambifaria AMMD

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q0BFH0
• Locus tag: CH72_RS16425
• Protein ID: WP_011656832.1
• Coordinates: 3528842..3529264 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q0BFH1
• Locus tag: CH72_RS16430
• Protein ID: WP_011656831.1
• Coordinates: 3529261..3529527 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH72_RS16405	3524944..3526014	+	1071	WP_011656836.1	hypothetical protein	-
CH72_RS16410	3525983..3526888	+	906	WP_011656835.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CH72_RS16415	3527176..3528228	-	1053	WP_011656834.1	transposase	-
CH72_RS16420	3528321..3528761	+	441	WP_011656833.1	MaoC family dehydratase	-
CH72_RS16425	3528842..3529264	-	423	WP_011656832.1	type II toxin-antitoxin system VapC family toxin	Toxin
CH72_RS16430	3529261..3529527	-	267	WP_011656831.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CH72_RS16435	3529805..3530821	-	1017	WP_011656830.1	acetyltransferase	-
CH72_RS16440	3530821..3531660	-	840	WP_006755049.1	folate-dependent phosphoribosylglycinamide formyltransferase PurN-like protein	-
CH72_RS16445	3531747..3534023	-	2277	WP_175783450.1	TonB-dependent siderophore receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14774.86 Da
• Isoelectric Point: 7.8895

>T49844 WP_011656832.1 NZ_CP009798:c3529264-3528842 [Burkholderia ambifaria AMMD]
VTTLYMLDTNICSFIMRERPSAVLSRLQSCVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVSRLDGVLSWDTA
AVDATTAIRVDLAARGTPIGANDASIAGHAIAAGAVLVTNNSREFSRVAGLSLEDWAASA

Nucleotide

• Length: 423 bp

>T49844 NZ_CP009798:c3529264-3528842 [Burkholderia ambifaria AMMD]
GTGACGACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCATCCGCGGTGTTGTCCCGGTT
GCAGTCGTGCGTCAATGCGCAGCACCGGATCGTCGTGTCGGCGATCACCTATGCCGAGATGCGATTCGGCGCAGTCGGCA
GAAAGGCTTCGCCGAAGCATGCGGAACTCGTGACGGCCTTTGTTTCGCGGCTGGATGGCGTGCTGTCGTGGGATACCGCG
GCGGTCGATGCAACGACCGCGATTCGCGTCGACCTTGCCGCACGTGGCACGCCGATCGGCGCGAACGACGCGTCGATCGC
CGGTCACGCGATCGCGGCCGGTGCCGTGCTTGTAACGAACAACAGTCGCGAATTCAGCCGTGTCGCGGGGTTGTCGCTCG
AAGATTGGGCAGCCTCGGCCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10017.29 Da
• Isoelectric Point: 4.5498

>AT49844 WP_011656831.1 NZ_CP009798:c3529527-3529261 [Burkholderia ambifaria AMMD]
MRTVSIFKNARNQAIRIPKDMEFEGVTELEIRREGDTLLLRPVRPTWLSFATEPRADADFLVERPTVVEAGRFELPGADD
DAQTESAR

Nucleotide

• Length: 267 bp

>AT49844 NZ_CP009798:c3529527-3529261 [Burkholderia ambifaria AMMD]
ATGCGCACTGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGTGTGAC
GGAACTCGAGATCCGCCGCGAGGGCGACACGTTGCTGCTGCGTCCGGTGCGGCCGACGTGGCTGTCGTTCGCGACTGAAC
CACGGGCCGATGCCGACTTCCTCGTCGAGCGCCCGACGGTCGTCGAAGCCGGTCGTTTCGAGCTGCCCGGCGCCGATGAC
GATGCACAGACGGAGTCGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: Q0BFH0

Antitoxin

• AlphaFold DB: Q0BFH1