Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 477137..477773 Replicon chromosome
Accession NZ_CP009796
Organism Bacillus subtilis strain SG6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag OB04_RS02460 Protein ID WP_003156187.1
Coordinates 477423..477773 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag OB04_RS02455 Protein ID WP_003225183.1
Coordinates 477137..477418 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OB04_RS02435 473497..474096 - 600 WP_038428385.1 rhomboid family intramembrane serine protease -
OB04_RS02440 474191..474556 + 366 WP_038428386.1 holo-ACP synthase -
OB04_RS02445 474722..475738 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
OB04_RS02450 475852..477021 + 1170 WP_015252766.1 alanine racemase -
OB04_RS02455 477137..477418 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
OB04_RS02460 477423..477773 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
OB04_RS02465 477889..478713 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
OB04_RS02470 478718..479083 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
OB04_RS02475 479087..479488 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
OB04_RS02480 479500..480507 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
OB04_RS02485 480569..480898 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
OB04_RS02490 480895..481377 + 483 WP_038427419.1 anti-sigma B factor RsbW -
OB04_RS02495 481343..482131 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
OB04_RS02500 482131..482730 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T49841 WP_003156187.1 NZ_CP009796:477423-477773 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T49841 NZ_CP009796:477423-477773 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT49841 WP_003225183.1 NZ_CP009796:477137-477418 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT49841 NZ_CP009796:477137-477418 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References