Detailed information of TA system TA049826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 26349..27051
• Replicon: plasmid pXF51ud
• Accession: NZ_CP009791
• Organism: Xylella fastidiosa strain U24d

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: XFUD_RS13120
• Protein ID: WP_042464090.1
• Coordinates: 26349..26735 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: XFUD_RS13125
• Protein ID: WP_023908247.1
• Coordinates: 26716..27051 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFUD_RS13075	21694..21966	-	273	WP_010895222.1	DUF4190 domain-containing protein	-
XFUD_RS13080	22031..22357	-	327	WP_010895223.1	type II toxin-antitoxin system PemK/MazF family toxin	-
XFUD_RS13085	22344..22574	-	231	WP_042464041.1	hypothetical protein	-
XFUD_RS13090	22854..23099	-	246	Protein_27	type II toxin-antitoxin system RelE/ParE family toxin	-
XFUD_RS13095	23143..23310	-	168	WP_042464043.1	type II toxin-antitoxin system RelE/ParE family toxin	-
XFUD_RS13100	23298..23551	-	254	Protein_29	CopG family ribbon-helix-helix protein	-
XFUD_RS13105	23811..24830	-	1020	WP_010895227.1	hypothetical protein	-
XFUD_RS13110	25191..25946	-	756	WP_010895228.1	alpha/beta hydrolase	-
XFUD_RS13115	26074..26238	-	165	Protein_32	recombinase family protein	-
XFUD_RS13120	26349..26735	+	387	WP_042464090.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XFUD_RS13125	26716..27051	+	336	WP_023908247.1	helix-turn-helix domain-containing protein	Antitoxin
XFUD_RS13130	27206..27805	-	600	WP_010895232.1	conjugal transfer protein TrbN	-
XFUD_RS13135	27811..29187	-	1377	WP_042463301.1	P-type conjugative transfer protein TrbL	-
XFUD_RS13140	29209..29445	-	237	WP_010894503.1	EexN family lipoprotein	-
XFUD_RS13145	29462..30235	-	774	WP_042464049.1	P-type conjugative transfer protein TrbJ	-
XFUD_RS13150	30254..31657	-	1404	WP_042464052.1	TrbI/VirB10 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..51156	51156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14170.35 Da
• Isoelectric Point: 9.7274

>T49826 WP_042464090.1 NZ_CP009791:26349-26735 [Xylella fastidiosa]
MPSWSIIEKPLEWIASSYKDLMALPPDVRRRFGYALSLAQRGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRTVYTVKFT
EAVFVLHCFQKKSKSGVATPKVDMNIIRARLKVAEALAQELRNAKTNH

Nucleotide

• Length: 387 bp

>T49826 NZ_CP009791:26349-26735 [Xylella fastidiosa]
ATGCCATCATGGTCGATAATAGAGAAACCGCTCGAATGGATCGCGAGCAGCTACAAGGATCTGATGGCGTTGCCGCCCGA
CGTGCGCCGGCGGTTTGGTTACGCGCTGTCGCTGGCACAGAGGGGCGACCAGGATGACGCGGCCAAGGTGCTCAAGGGCT
TCGGCGGGGCGGGCGTACTGGAAGTCGTCGAAGACGATGCCGGTGGCACGTACCGGACGGTCTACACGGTGAAGTTCACG
GAAGCCGTGTTCGTCCTGCATTGCTTCCAGAAGAAGAGCAAGAGCGGGGTCGCCACGCCAAAGGTGGACATGAACATCAT
CCGTGCTCGGCTGAAGGTAGCCGAGGCACTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12265.39 Da
• Isoelectric Point: 10.0997

>AT49826 WP_023908247.1 NZ_CP009791:26716-27051 [Xylella fastidiosa]
MQKRIIEGVEVQRSSGNVFADLGLHDAEKLKIKTGLVIEIRKAMHALGLNQQEAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPVGHLTLATA

Nucleotide

• Length: 336 bp

>AT49826 NZ_CP009791:26716-27051 [Xylella fastidiosa]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAACGCAGCTCGGGCAACGTCTTCGCCGACTTGGGACTGCATGACGC
CGAAAAACTCAAGATCAAGACCGGGCTGGTGATCGAGATCAGGAAGGCGATGCACGCCCTCGGGCTGAATCAGCAAGAGG
CGGCGAAACGCATGGGTATCCCGCAACCGAAGGTGTCGGGCATGATGCGTGGCGACTTCACCAACTTGTCCGAACGCAAG
CTGATGGACTGCCTGAATCGCCTCGGCTACGACATCGAGATCAAGGTACGGCCGGCCGCTGAGCCGGTCGGGCATCTGAC
GCTCGCAACCGCATGA