Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1489716..1490354 Replicon chromosome
Accession NZ_CP009789
Organism Escherichia coli K-12 strain K-12 ER3413

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ER3413_RS07440 Protein ID WP_000813794.1
Coordinates 1489716..1489892 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ER3413_RS07445 Protein ID WP_001270286.1
Coordinates 1489938..1490354 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ER3413_RS07420 1485335..1486549 - 1215 WP_001326689.1 BenE family transporter YdcO -
ER3413_RS07425 1486602..1487138 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
ER3413_RS07430 1487211..1489172 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ER3413_RS07435 1489264..1489494 - 231 WP_000494244.1 YncJ family protein -
ER3413_RS07440 1489716..1489892 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ER3413_RS07445 1489938..1490354 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ER3413_RS07450 1490433..1491839 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
ER3413_RS07455 1492084..1493229 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
ER3413_RS07460 1493247..1494260 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
ER3413_RS07465 1494261..1495202 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T49789 WP_000813794.1 NZ_CP009789:1489716-1489892 [Escherichia coli K-12]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T49789 NZ_CP009789:1489716-1489892 [Escherichia coli K-12]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT49789 WP_001270286.1 NZ_CP009789:1489938-1490354 [Escherichia coli K-12]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT49789 NZ_CP009789:1489938-1490354 [Escherichia coli K-12]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References