Detailed information of TA system TA049778

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yfjZ/CbtA-CbeA
• Location: 4136845..4137545
• Replicon: chromosome
• Accession: NZ_CP009787
• Organism: Yersinia rohdei strain YRA

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CH64_RS18150
• Protein ID: WP_004714666.1
• Coordinates: 4136845..4137216 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CH64_RS18155
• Protein ID: WP_032816883.1
• Coordinates: 4137213..4137545 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH64_RS18125	4132780..4133571	+	792	WP_042542395.1	50S ribosome-binding GTPase	-
CH64_RS18130	4133821..4134375	+	555	WP_032816892.1	hypothetical protein	-
CH64_RS18135	4134612..4134890	+	279	WP_200891557.1	hypothetical protein	-
CH64_RS18140	4135146..4135967	+	822	WP_032816882.1	DUF945 domain-containing protein	-
CH64_RS18145	4136322..4136753	+	432	WP_004714665.1	antirestriction protein	-
CH64_RS18150	4136845..4137216	+	372	WP_004714666.1	TA system toxin CbtA family protein	Toxin
CH64_RS18155	4137213..4137545	+	333	WP_032816883.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CH64_RS18160	4137631..4138485	+	855	WP_004714667.1	DUF4942 domain-containing protein	-
CH64_RS18165	4138648..4139652	-	1005	WP_042542338.1	site-specific integrase	-
CH64_RS18170	4139587..4140051	+	465	WP_082044792.1	type II toxin-antitoxin system HicB family antitoxin	-
CH64_RS18175	4140431..4141066	+	636	WP_032818540.1	DUF1120 domain-containing protein	-
CH64_RS18180	4141330..4141527	+	198	WP_032818541.1	phage holin family protein	-
CH64_RS18185	4141558..4141815	+	258	Protein_3565	lysozyme	-
CH64_RS20345	4141884..4142045	+	162	Protein_3566	baseplate J/gp47 family protein	-
CH64_RS18190	4142057..4142476	+	420	WP_004716911.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	yapC	4111535..4176686	65151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (14-99)

Antitoxin

• Antitoxin CbeA (10-108)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13586.68 Da
• Isoelectric Point: 6.0574

>T49778 WP_004714666.1 NZ_CP009787:4136845-4137216 [Yersinia rohdei]
MKPCPITLGALPVPVIYQQLLAFLLEHHYGLSLNDTAYHDEQLIVQQIEQGISVGDTINTLVEKYALVRIGRNGFSALAQ
DPTLSKGDIARARQAAGLWCAHFPPTLLYPTEPLNPIPQRELT

Nucleotide

• Length: 372 bp

>T49778 NZ_CP009787:4136845-4137216 [Yersinia rohdei]
ATGAAACCATGTCCCATCACACTGGGCGCATTACCCGTGCCTGTTATTTATCAACAACTGCTCGCTTTCCTGCTTGAGCA
CCACTATGGCCTGAGCCTCAACGATACGGCCTATCACGATGAACAGTTGATTGTGCAGCAGATAGAACAAGGGATTTCAG
TGGGCGACACCATCAACACACTGGTTGAGAAATATGCCTTAGTGCGAATTGGTCGTAACGGGTTCAGCGCACTGGCACAA
GACCCGACGCTGAGTAAGGGCGATATCGCCCGTGCTCGCCAGGCCGCAGGTCTGTGGTGTGCGCATTTTCCGCCAACGTT
GCTGTACCCAACCGAGCCCCTCAATCCTATTCCACAGAGAGAGTTAACATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12116.75 Da
• Isoelectric Point: 6.4753

>AT49778 WP_032816883.1 NZ_CP009787:4137213-4137545 [Yersinia rohdei]
MNNHITISPIWGLKRDITPRLGARLVQEGANLHFLSDRANIVGTFSPQATQALDAAFPELITKLESQLLSGALDPRRQQG
ITVRHGEFVCEADTLGSHGYVYIAVYQELS

Nucleotide

• Length: 333 bp

>AT49778 NZ_CP009787:4137213-4137545 [Yersinia rohdei]
ATGAATAACCACATTACTATTTCCCCAATATGGGGCCTGAAACGTGATATTACGCCACGTTTAGGTGCCAGGCTGGTACA
GGAGGGAGCAAACCTGCACTTTCTGAGTGACAGAGCCAATATTGTCGGTACCTTTTCCCCTCAAGCCACTCAGGCTCTTG
ATGCTGCATTTCCAGAGCTGATAACCAAACTGGAGTCACAGCTGCTGTCCGGGGCGCTGGATCCACGCCGTCAGCAAGGC
ATTACGGTTCGGCATGGAGAATTTGTCTGTGAAGCGGATACGCTGGGCAGTCACGGTTATGTCTACATCGCGGTGTATCA
GGAACTATCTTGA