Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4052855..4053474 Replicon chromosome
Accession NZ_CP009787
Organism Yersinia rohdei strain YRA

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag CH64_RS17800 Protein ID WP_002208622.1
Coordinates 4052855..4053058 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0U1HRM3
Locus tag CH64_RS17805 Protein ID WP_032816856.1
Coordinates 4053106..4053474 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH64_RS17775 4048045..4048383 + 339 WP_002208627.1 P-II family nitrogen regulator -
CH64_RS17780 4048423..4049712 + 1290 WP_032816887.1 ammonium transporter AmtB -
CH64_RS17785 4049898..4050764 - 867 WP_004714595.1 acyl-CoA thioesterase II -
CH64_RS17790 4051012..4051536 + 525 WP_004714596.1 YbaY family lipoprotein -
CH64_RS17795 4051582..4051890 - 309 WP_145522794.1 MGMT family protein -
CH64_RS17800 4052855..4053058 - 204 WP_002208622.1 expression modulating protein YmoA Toxin
CH64_RS17805 4053106..4053474 - 369 WP_032816856.1 Hha toxicity modulator TomB Antitoxin
CH64_RS17810 4053633..4053986 - 354 WP_004714600.1 hypothetical protein -
CH64_RS17815 4054738..4055469 + 732 WP_072086988.1 ABC transporter ATP-binding protein -
CH64_RS17820 4055469..4056326 + 858 WP_004714601.1 metal ABC transporter permease -
CH64_RS17825 4056359..4057240 + 882 WP_004714602.1 metal ABC transporter substrate-binding protein -
CH64_RS17830 4057360..4057503 - 144 WP_002208618.1 type B 50S ribosomal protein L36 -
CH64_RS17835 4057519..4057779 - 261 WP_004714603.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T49777 WP_002208622.1 NZ_CP009787:c4053058-4052855 [Yersinia rohdei]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T49777 NZ_CP009787:c4053058-4052855 [Yersinia rohdei]
ATGACAAAGACTGACTATCTAATGCGTTTAAGAAAATGTACGACTATCGATACATTAGAGCGCGTAATTGAAAAAAATAA
GTATGAGCTTTCAGATGATGAGTTGGAATTATTTTACTCAGCCGCTGACCATCGTTTAGCTGAACTCACTATGAATAAGC
TTTATGATAAAATTCCCCCTACCGTCTGGCAACATGTAAAATAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14171.93 Da        Isoelectric Point: 4.5221

>AT49777 WP_032816856.1 NZ_CP009787:c4053474-4053106 [Yersinia rohdei]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDGGELSELVEEYL
DDTYTLFSSYGINDPELHRWQKTKERLFRLFSGEYICTLMKT

Download         Length: 369 bp

>AT49777 NZ_CP009787:c4053474-4053106 [Yersinia rohdei]
ATGGATGAGTACTCACCTAAGCGGCATGATATTGCCCAATTGAAGTTTCTATGTGAGAGTTTGTATGATGAAGGTATCGC
CACCCTCGGTGATAGTCACCATGGCTGGGTAAATGACCCGACATCTGCCGTAAACCTGCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCCGATGGCGGTGAACTGTCAGAGTTAGTTGAAGAATATTTA
GACGATACATATACTTTGTTTAGTAGTTATGGGATCAACGATCCTGAATTGCACCGCTGGCAAAAAACTAAAGAGCGTTT
ATTCAGACTATTCTCAGGGGAATACATCTGTACCCTGATGAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2K5S

Antitoxin

Source ID Structure
AlphaFold DB A0A0U1HRM3

References