Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 36991..37727 Replicon plasmid pKPN-a68
Accession NZ_CP009777
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH32

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KPNIH32_RS28550 Protein ID WP_003026803.1
Coordinates 36991..37473 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KPNIH32_RS28555 Protein ID WP_003026799.1
Coordinates 37461..37727 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH32_RS28530 32628..33590 + 963 WP_004152113.1 M48 family metalloprotease -
KPNIH32_RS32180 33678..34932 + 1255 Protein_40 IS3 family transposase -
KPNIH32_RS28545 35433..36779 - 1347 WP_020314316.1 ISNCY family transposase -
KPNIH32_RS28550 36991..37473 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KPNIH32_RS28555 37461..37727 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KPNIH32_RS28560 37903..38157 - 255 WP_004152108.1 hypothetical protein -
KPNIH32_RS28565 38233..38490 - 258 WP_004152107.1 hypothetical protein -
KPNIH32_RS28570 38539..38742 - 204 WP_004152106.1 hemolysin expression modulator Hha -
KPNIH32_RS28575 38776..39144 - 369 WP_004152105.1 hypothetical protein -
KPNIH32_RS28580 39188..39682 - 495 WP_004152104.1 DNA-binding protein -
KPNIH32_RS28585 39713..40288 - 576 WP_004152103.1 hypothetical protein -
KPNIH32_RS28590 40276..40545 - 270 WP_004152102.1 hypothetical protein -
KPNIH32_RS28595 40903..41253 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KPNIH32_RS28600 41303..41665 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia / catA1 - 1..212192 212192


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T49706 WP_003026803.1 NZ_CP009777:c37473-36991 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T49706 NZ_CP009777:c37473-36991 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT49706 WP_003026799.1 NZ_CP009777:c37727-37461 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT49706 NZ_CP009777:c37727-37461 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References