Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 111198..111841 Replicon plasmid pKPC-def
Accession NZ_CP009776
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH32

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KPNIH32_RS28305 Protein ID WP_001044770.1
Coordinates 111198..111614 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KPNIH32_RS28310 Protein ID WP_001261282.1
Coordinates 111611..111841 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH32_RS28260 106492..106776 - 285 WP_001043731.1 hypothetical protein -
KPNIH32_RS28265 106812..107123 - 312 WP_000469598.1 hypothetical protein -
KPNIH32_RS28270 107329..107613 - 285 WP_001185481.1 hypothetical protein -
KPNIH32_RS28275 107713..108375 - 663 WP_000516402.1 peptidyl-arginine deiminase -
KPNIH32_RS28280 108756..109418 + 663 WP_001549893.1 recombinase family protein -
KPNIH32_RS28285 109505..109744 + 240 WP_001549892.1 hypothetical protein -
KPNIH32_RS28295 110137..110841 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
KPNIH32_RS28300 110888..111124 - 237 WP_001348075.1 AAA family ATPase -
KPNIH32_RS28305 111198..111614 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KPNIH32_RS28310 111611..111841 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KPNIH32_RS31035 111798..112258 + 461 Protein_134 hypothetical protein -
KPNIH32_RS32375 112386..112706 + 321 WP_032072060.1 hypothetical protein -
KPNIH32_RS28315 112755..113393 + 639 WP_022644887.1 hypothetical protein -
KPNIH32_RS28320 113396..114181 + 786 WP_022644886.1 site-specific integrase -
KPNIH32_RS28325 114239..114496 - 258 WP_032408936.1 hypothetical protein -
KPNIH32_RS32995 114625..114738 - 114 WP_032072094.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / blaSHV-12 - 1..115320 115320
- flank IS/Tn - - 110137..110841 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T49705 WP_001044770.1 NZ_CP009776:c111614-111198 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T49705 NZ_CP009776:c111614-111198 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT49705 WP_001261282.1 NZ_CP009776:c111841-111611 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT49705 NZ_CP009776:c111841-111611 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References