Detailed information of TA system TA049686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5819..6344
• Replicon: plasmid pNJST258N3-62b
• Accession: NZ_CP009774
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPNIH33

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: W8UQV6
• Locus tag: KPNIH33_RS28425
• Protein ID: WP_001568026.1
• Coordinates: 5819..6124 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: KPNIH33_RS28430
• Protein ID: WP_001568025.1
• Coordinates: 6126..6344 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPNIH33_RS28395	1519..2145	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
KPNIH33_RS30320	2265..2444	+	180	WP_006788217.1	hypothetical protein	-
KPNIH33_RS28405	2900..3694	-	795	WP_004197635.1	site-specific integrase	-
KPNIH33_RS28410	3892..4908	-	1017	WP_020315256.1	hypothetical protein	-
KPNIH33_RS28415	4905..5228	-	324	WP_022644730.1	hypothetical protein	-
KPNIH33_RS28420	5255..5650	-	396	WP_017899885.1	hypothetical protein	-
KPNIH33_RS28425	5819..6124	-	306	WP_001568026.1	type II toxin-antitoxin system toxin CcdB	Toxin
KPNIH33_RS28430	6126..6344	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KPNIH33_RS28435	6897..7586	+	690	WP_004193995.1	hypothetical protein	-
KPNIH33_RS28440	7618..8307	-	690	WP_020315560.1	RES family NAD+ phosphorylase	-
KPNIH33_RS28445	8744..9013	+	270	WP_020804424.1	hypothetical protein	-
KPNIH33_RS28450	9001..9576	+	576	WP_020317495.1	hypothetical protein	-
KPNIH33_RS28455	9607..10101	+	495	WP_020802777.1	hypothetical protein	-
KPNIH33_RS28460	10145..10513	+	369	WP_004197807.1	hypothetical protein	-
KPNIH33_RS28465	10547..10750	+	204	WP_004197808.1	hemolysin expression modulator Hha	-
KPNIH33_RS28595	10764..10967	+	204	WP_004197809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36109	36109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11645.32 Da
• Isoelectric Point: 5.6919

>T49686 WP_001568026.1 NZ_CP009774:c6124-5819 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T49686 NZ_CP009774:c6124-5819 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT49686 WP_001568025.1 NZ_CP009774:c6344-6126 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT49686 NZ_CP009774:c6344-6126 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A514EZN1

Antitoxin

• AlphaFold DB: A0A2J4ZZP8