49685

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 71496..72139 Replicon plasmid pKPC-63d
Accession NZ_CP009773
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH33

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KPNIH33_RS28360 Protein ID WP_001044770.1
Coordinates 71496..71912 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KPNIH33_RS28365 Protein ID WP_001261282.1
Coordinates 71909..72139 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH33_RS31655 67387..67452 + 66 Protein_71 EAL domain-containing protein -
KPNIH33_RS28330 67592..68356 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
KPNIH33_RS28335 68499..68765 - 267 WP_001144737.1 plasmid mobilization relaxosome protein MobC -
KPNIH33_RS28340 68986..69459 - 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
KPNIH33_RS28345 69615..70382 + 768 Protein_75 class 1 integron integrase IntI1 -
KPNIH33_RS28350 70435..71139 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
KPNIH33_RS28355 71186..71422 - 237 WP_001348075.1 AAA family ATPase -
KPNIH33_RS28360 71496..71912 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KPNIH33_RS28365 71909..72139 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KPNIH33_RS30025 72096..72556 + 461 Protein_80 hypothetical protein -
KPNIH33_RS31030 72684..73004 + 321 WP_032072060.1 hypothetical protein -
KPNIH33_RS28370 73053..73691 + 639 WP_022644887.1 hypothetical protein -
KPNIH33_RS28375 73694..74479 + 786 WP_022644886.1 site-specific integrase -
KPNIH33_RS28380 74537..74794 - 258 WP_032408936.1 hypothetical protein -
KPNIH33_RS31660 74923..75036 - 114 WP_032072094.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..75618 75618


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T49685 WP_001044770.1 NZ_CP009773:c71912-71496 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T49685 NZ_CP009773:c71912-71496 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT49685 WP_001261282.1 NZ_CP009773:c72139-71909 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT49685 NZ_CP009773:c72139-71909 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References