Detailed information of TA system TA049660

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3387554..3388279
• Replicon: chromosome
• Accession: NZ_CP009769
• Organism: Pectobacterium brasiliense strain BC1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: NC16_RS15410
• Protein ID: WP_039503067.1
• Coordinates: 3387959..3388279 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: NC16_RS15405
• Protein ID: WP_039503065.1
• Coordinates: 3387554..3387889 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NC16_RS15370	3382738..3383613	+	876	WP_039503057.1	GTPase family protein	-
NC16_RS15375	3383698..3384519	+	822	WP_039503058.1	DUF945 domain-containing protein	-
NC16_RS15380	3384745..3385440	+	696	Protein_2958	DeoR family transcriptional regulator	-
NC16_RS15385	3385625..3386194	+	570	WP_039308205.1	hypothetical protein	-
NC16_RS15390	3386230..3386703	+	474	WP_039308202.1	hypothetical protein	-
NC16_RS15395	3386720..3386953	+	234	WP_039503061.1	membrane protein	-
NC16_RS15400	3387041..3387514	+	474	WP_039503063.1	DNA repair protein RadC	-
NC16_RS15405	3387554..3387889	+	336	WP_039503065.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
NC16_RS15410	3387959..3388279	+	321	WP_039503067.1	TA system toxin CbtA family protein	Toxin
NC16_RS15415	3388392..3389225	+	834	WP_039503070.1	DUF4942 domain-containing protein	-
NC16_RS15420	3389651..3390469	-	819	WP_131547287.1	hypothetical protein	-
NC16_RS15425	3390916..3391173	+	258	Protein_2967	DUF4942 domain-containing protein	-
NC16_RS15430	3391267..3391497	+	231	WP_039360473.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
NC16_RS15435	3391497..3391901	+	405	WP_039503077.1	tRNA(fMet)-specific endonuclease VapC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3372990..3399903	26913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12194.02 Da
• Isoelectric Point: 4.7768

>T49660 WP_039503067.1 NZ_CP009769:3387959-3388279 [Pectobacterium brasiliense]
MQTTPAIPLREDQSCPPPIAVWQQLLTYLLEKHYGLTLNDTPFCEENVIQEHIDAGITLVNAINFLVEKYELVRIDRNGF
NWQEQSPFLTTVDILRARRVTGLLKA

Nucleotide

• Length: 321 bp

>T49660 NZ_CP009769:3387959-3388279 [Pectobacterium brasiliense]
ATGCAAACAACACCTGCAATCCCGTTACGGGAGGATCAATCCTGCCCGCCACCCATAGCCGTCTGGCAGCAACTTCTGAC
CTATCTGTTGGAAAAGCACTACGGCCTCACGCTGAATGACACACCATTCTGCGAGGAGAACGTGATTCAGGAGCATATCG
ATGCTGGCATCACGTTGGTAAATGCCATCAATTTTCTGGTGGAAAAATACGAACTGGTGCGCATTGATCGCAACGGTTTT
AACTGGCAGGAGCAATCACCGTTTCTCACGACTGTCGATATTCTTCGCGCCAGACGTGTAACAGGCTTACTTAAAGCATA
A

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12517.17 Da
• Isoelectric Point: 6.4800

>AT49660 WP_039503065.1 NZ_CP009769:3387554-3387889 [Pectobacterium brasiliense]
MSSIEAPEWGLKRAVTPQFGARLVQEGHYLHYLADRASVVGTFSKTEAHHLEQIFPELIKQLEQKLRSGELNPRQQGCIT
LHCNEWTCEADTLGSFGYVYIVIYPTSAATH

Nucleotide

• Length: 336 bp

>AT49660 NZ_CP009769:3387554-3387889 [Pectobacterium brasiliense]
ATGTCATCAATAGAGGCCCCGGAATGGGGACTAAAACGTGCTGTCACGCCGCAATTCGGAGCCAGACTGGTTCAGGAAGG
TCACTATCTTCATTATCTGGCAGACCGGGCCAGCGTTGTCGGTACGTTCAGCAAAACGGAAGCCCATCATCTGGAACAGA
TCTTTCCTGAGCTGATTAAGCAGTTGGAACAAAAACTGCGCTCGGGGGAACTGAATCCTCGCCAGCAGGGCTGCATTACG
CTGCATTGCAATGAATGGACCTGTGAAGCCGATACGCTGGGCAGTTTTGGTTATGTGTATATTGTTATTTATCCCACATC
TGCTGCAACACACTAA