Detailed information of TA system TA049659

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3097238..3097962
• Replicon: chromosome
• Accession: NZ_CP009769
• Organism: Pectobacterium brasiliense strain BC1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: NC16_RS14120
• Protein ID: WP_039503572.1
• Coordinates: 3097238..3097558 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: NC16_RS14125
• Protein ID: WP_039503575.1
• Coordinates: 3097627..3097962 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NC16_RS14090	3092507..3092725	+	219	WP_010279476.1	KTSC domain-containing protein	-
NC16_RS14095	3092950..3093306	+	357	WP_039503564.1	hypothetical protein	-
NC16_RS14100	3093361..3094260	-	900	WP_039503565.1	LysR family transcriptional regulator	-
NC16_RS14105	3094372..3095385	+	1014	WP_039503567.1	CAR family subclass B3 metallo-beta-lactamase	-
NC16_RS14110	3095959..3096156	-	198	WP_052325999.1	hypothetical protein	-
NC16_RS14115	3096286..3097119	-	834	WP_039503570.1	DUF4942 domain-containing protein	-
NC16_RS14120	3097238..3097558	-	321	WP_039503572.1	TA system toxin CbtA family protein	Toxin
NC16_RS14125	3097627..3097962	-	336	WP_039503575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
NC16_RS14130	3098002..3098475	-	474	WP_039503577.1	DNA repair protein RadC	-
NC16_RS14135	3098682..3099287	-	606	WP_039503579.1	hypothetical protein	-
NC16_RS14140	3099316..3099870	-	555	WP_039503580.1	hypothetical protein	-
NC16_RS14145	3100169..3101044	-	876	WP_080752722.1	50S ribosome-binding GTPase	-
NC16_RS14150	3101118..3101975	-	858	WP_039503583.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	flhA / flhB / cheZ / cheY / cheB / cheR / cheD / cheW / cheA / motB / motA / flhC / flhD / hcp	3071656..3144881	73225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (8-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12079.83 Da
• Isoelectric Point: 4.7768

>T49659 WP_039503572.1 NZ_CP009769:c3097558-3097238 [Pectobacterium brasiliense]
MQTSPATPPREDNPCALPIAVWQQLLTYLLEKHYGLTLNDTPFCEENVIQEHIDAGITLVNAVNFLVEKYELVRIDRNGF
NWQEQSPFLTAVDVLRARRATGLLKV

Nucleotide

• Length: 321 bp

>T49659 NZ_CP009769:c3097558-3097238 [Pectobacterium brasiliense]
ATGCAAACATCACCTGCAACCCCGCCACGGGAGGATAACCCCTGTGCGTTACCTATAGCCGTCTGGCAACAACTGCTGAC
CTACCTGCTGGAAAAGCACTACGGCCTGACGTTGAATGACACACCGTTCTGTGAGGAAAACGTGATTCAGGAGCATATCG
ATGCTGGCATCACGTTGGTCAATGCCGTCAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGATCGCAACGGCTTT
AACTGGCAGGAGCAATCGCCATTTCTCACTGCTGTTGATGTTCTCCGTGCCAGACGCGCTACTGGCTTGCTCAAAGTATA
A

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12314.84 Da
• Isoelectric Point: 5.1308

>AT49659 WP_039503575.1 NZ_CP009769:c3097962-3097627 [Pectobacterium brasiliense]
MPSSDTSEWGLKCAITPRFGARLVQEGNRLHYLADRASVVGTFSKTEAQSLERCFSELIKQLEQKLRTGELNPRQQGGVT
LYCNEWTCEADTLGSFGYVYIAIYPSSAAAE

Nucleotide

• Length: 336 bp

>AT49659 NZ_CP009769:c3097962-3097627 [Pectobacterium brasiliense]
ATGCCATCATCGGATACCTCTGAATGGGGATTAAAATGTGCCATCACGCCGCGATTTGGAGCCAGGCTGGTGCAGGAAGG
TAACCGTCTGCATTATCTGGCAGACCGGGCCAGCGTTGTCGGTACGTTCAGCAAAACGGAAGCCCAAAGTCTGGAACGTT
GCTTCTCTGAGCTGATCAAGCAGTTGGAGCAGAAACTACGCACGGGGGAACTGAATCCTCGCCAGCAGGGGGGTGTCACG
TTGTATTGCAACGAATGGACCTGTGAAGCCGATACGCTGGGCAGTTTTGGCTACGTGTATATTGCTATTTATCCCTCTTC
TGCGGCAGCAGAATAA