Detailed information of TA system TA049657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 964828..965333
• Replicon: chromosome
• Accession: NZ_CP009769
• Organism: Pectobacterium brasiliense strain BC1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: NC16_RS04400
• Protein ID: WP_039502695.1
• Coordinates: 964828..965112 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: NC16_RS04405
• Protein ID: WP_039502698.1
• Coordinates: 965115..965333 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NC16_RS04370	960900..961379	+	480	WP_039502689.1	hypothetical protein	-
NC16_RS04380	961685..962428	-	744	WP_052325987.1	hypothetical protein	-
NC16_RS04385	962832..963056	+	225	WP_039502691.1	DUF1778 domain-containing protein	-
NC16_RS04390	963053..963547	+	495	Protein_843	GNAT family N-acetyltransferase	-
NC16_RS04395	963596..964405	+	810	WP_039502693.1	hypothetical protein	-
NC16_RS04400	964828..965112	-	285	WP_039502695.1	type II toxin-antitoxin system toxin CcdB	Toxin
NC16_RS04405	965115..965333	-	219	WP_039502698.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
NC16_RS04410	965348..965596	-	249	WP_039502700.1	helix-turn-helix domain-containing protein	-
NC16_RS04415	965681..965935	-	255	WP_039285388.1	helix-turn-helix domain-containing protein	-
NC16_RS04420	966039..966281	+	243	WP_072036092.1	helix-turn-helix domain-containing protein	-
NC16_RS04430	967109..969262	+	2154	WP_039502702.1	ornithine decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-93)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11063.82 Da
• Isoelectric Point: 5.6031

>T49657 WP_039502695.1 NZ_CP009769:c965112-964828 [Pectobacterium brasiliense]
MQFVVYQYKHVSNYKMFVDVQSDIVETPKRRMVIPLIESHHLSEKVNKTLFPQIRINGEDYRLMTTELSSVPVEVIGEYA
DEIKDAINLMFWGI

Nucleotide

• Length: 285 bp

>T49657 NZ_CP009769:c965112-964828 [Pectobacterium brasiliense]
ATGCAATTCGTTGTTTATCAATACAAACACGTCAGTAACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGCATGGTCATTCCGCTTATCGAATCACATCACCTGTCTGAGAAAGTGAATAAGACGCTGTTCCCTCAGA
TCCGCATCAACGGCGAAGATTATCGATTGATGACCACTGAGCTATCGAGCGTTCCCGTTGAGGTTATTGGCGAGTATGCC
GATGAGATAAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8250.32 Da
• Isoelectric Point: 10.0902

>AT49657 WP_039502698.1 NZ_CP009769:c965333-965115 [Pectobacterium brasiliense]
MKRRISIAVDKNDNQIPNATGIPISGLVNNVIGKEVRRIKTEEWKKENREGMENVAQFIAQHGSFADENRTW

Nucleotide

• Length: 219 bp

>AT49657 NZ_CP009769:c965333-965115 [Pectobacterium brasiliense]
ATGAAACGCCGGATTAGCATTGCCGTGGACAAAAACGATAATCAGATTCCCAATGCTACCGGGATACCTATTTCTGGTCT
GGTGAATAACGTCATAGGCAAAGAAGTCCGCCGTATCAAAACTGAAGAGTGGAAGAAGGAAAATCGCGAAGGGATGGAAA
ACGTCGCCCAATTTATCGCACAGCATGGTTCCTTTGCGGATGAAAACAGGACCTGGTGA