Detailed information of TA system TA049655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 526735..527519
• Replicon: chromosome
• Accession: NZ_CP009769
• Organism: Pectobacterium brasiliense strain BC1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: NC16_RS02400
• Protein ID: WP_039504316.1
• Coordinates: 527025..527519 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: NC16_RS02395
• Protein ID: WP_039504314.1
• Coordinates: 526735..527028 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NC16_RS02370	522344..522604	-	261	WP_039504301.1	helix-turn-helix domain-containing protein	-
NC16_RS02375	522828..524105	-	1278	WP_131547298.1	hypothetical protein	-
NC16_RS02380	524346..524606	-	261	WP_039504308.1	helix-turn-helix domain-containing protein	-
NC16_RS02385	524727..524969	+	243	WP_039504310.1	helix-turn-helix domain-containing protein	-
NC16_RS02390	525046..526362	-	1317	WP_039526449.1	IS4 family transposase	-
NC16_RS02395	526735..527028	+	294	WP_039504314.1	DUF1778 domain-containing protein	Antitoxin
NC16_RS02400	527025..527519	+	495	WP_039504316.1	GNAT family N-acetyltransferase	Toxin
NC16_RS02405	527585..527823	-	239	Protein_460	transposase	-
NC16_RS02410	527921..529756	-	1836	WP_039504318.1	protein kinase	-
NC16_RS02415	530137..530883	-	747	WP_039504320.1	hypothetical protein	-
NC16_RS22565	531025..531195	+	171	WP_157891289.1	hypothetical protein	-
NC16_RS02425	531787..532128	-	342	WP_039504322.1	hypothetical protein	-
NC16_RS02430	532314..532508	+	195	WP_012773549.1	DUF3950 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	526478..534043	7565
	-	flank	IS/Tn	-	-	525046..526362	1316

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17695.58 Da
• Isoelectric Point: 7.2922

>T49655 WP_039504316.1 NZ_CP009769:527025-527519 [Pectobacterium brasiliense]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVSGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKLLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T49655 NZ_CP009769:527025-527519 [Pectobacterium brasiliense]
ATGATCTCTGCCCCTGAGCCACTCCACGCCGAACATGTACTATCCTCATTCTGTTGTGGCGTGGAGTCTATGGACAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTATCTGGCGCATCACGGACTTTCGTTAGTTGCGATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGTGCCGTGGCAACGAATGCCGCTCCCGGACGATTCCGCCGCAATATGCCCGATCCGATC
CCGGTCGTGGTGCTGGGTCGTCTGGCAGTAGATAAATTATTGCATGGTCAGGGTGTTGGCCGTGCTCTGGTACGTGATGC
AGGGCTAAGAGTGATTCAGGTTGCTGATACTATCGGCATTCGCGGGATGTTGGTTCATGCGTTGTCTGATGAAGCGCGGG
AATTTTACCTACGTGTGGGGTTTGAGCCTTCGCCGATAGATCCGATGATGTTGATGGTGACGCTGGGGGATTTGGTGGGG
AGTTTATCGATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10886.51 Da
• Isoelectric Point: 10.1968

>AT49655 WP_039504314.1 NZ_CP009769:526735-527028 [Pectobacterium brasiliense]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTLAPWEQKK

Nucleotide

• Length: 294 bp

>AT49655 NZ_CP009769:526735-527028 [Pectobacterium brasiliense]
ATGCCAGCGACGAACAGCGTTTCTGTTAAACGCGAAACATTGAATTTACGCATCAAACCCGCAGAACGGGATCTGATTGA
TCGTGCAGCGAAGGCCAGAGGAAAAAATCGGACTGATTTTGTCCTTGAGGCCGCGCGTGCTGCCGCAGAGGAAGCGTTGA
TCGAACAACGCATCATCATGGCAGATCCCGACGCTTATCAGGAATTCCTTGCCCGTTTGGATCAGGCTCCTGCACCAAAT
GCCGCGCTGCGTAAAACCATGCAAACACTTGCACCGTGGGAGCAGAAAAAATGA