Detailed information of TA system TA049652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 147706..148340
• Replicon: chromosome
• Accession: NZ_CP009769
• Organism: Pectobacterium brasiliense strain BC1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: NC16_RS00655
• Protein ID: WP_080162911.1
• Coordinates: 147706..147981 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: NC16_RS00660
• Protein ID: WP_014917022.1
• Coordinates: 147978..148340 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NC16_RS00625	143153..143851	-	699	WP_010281574.1	envelope stress response regulator transcription factor CpxR	-
NC16_RS00630	144005..144529	+	525	WP_039502366.1	cell-envelope stress modulator CpxP	-
NC16_RS00640	145146..145328	+	183	Protein_124	hypothetical protein	-
NC16_RS00645	145486..147144	+	1659	WP_039502364.1	hypothetical protein	-
NC16_RS00650	147131..147337	+	207	WP_039502363.1	hypothetical protein	-
NC16_RS00655	147706..147981	+	276	WP_080162911.1	type II toxin-antitoxin system HicA family toxin	Toxin
NC16_RS00660	147978..148340	+	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NC16_RS00665	148714..149901	+	1188	WP_039502362.1	sugar transporter	-
NC16_RS00670	149984..150886	+	903	WP_039502361.1	CDF family cation-efflux transporter FieF	-
NC16_RS00675	151094..152056	+	963	WP_010306854.1	6-phosphofructokinase	-
NC16_RS00680	152337..153221	+	885	WP_039465389.1	N-carbamoylputrescine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	145486..155064	9578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10024.61 Da
• Isoelectric Point: 11.1875

>T49652 WP_080162911.1 NZ_CP009769:147706-147981 [Pectobacterium brasiliense]
MQGKIASLRKKQRETLSQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T49652 NZ_CP009769:147706-147981 [Pectobacterium brasiliense]
ATGCAGGGAAAAATAGCCAGCCTACGAAAGAAGCAGCGGGAAACGCTGAGCCAAATTTTCAAAACACCAGTACTTTCAGG
TGTTAAATGGTCGAATATTGAGTCACTCGTTACTGCGTTAGGCGGTGAAATTAAGGAAGGTAGCGGATCACGAGTGCGAT
TTTTATTGAACGGGAGTATCGCCCGATTCCATCGTCCTCATCCGTCACCGGATACTGATAAAGGCGCACTGGTTAGCCTG
CGTGAATGGCTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT49652 WP_014917022.1 NZ_CP009769:147978-148340 [Pectobacterium brasiliense]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT49652 NZ_CP009769:147978-148340 [Pectobacterium brasiliense]
ATGACGAAAGCACTCAACACACCAAATACGATGGTTGTTTCTGGACAACCGGCCATTATCAGTTATGTACCGGAAATGGG
TATGTTTCGCGGTAAGTTTATTGGTTTATCTGGCTACTGTGATTTTGTCGCCGACAGTATTAATGGCTTGAGAAGCGAGG
GTGAAATTTCGCTACGCGAATATCTGGAAGACTGTCAGGAAAATGGCATTGAACCTTACGAGCGTGAAGAGAAGGTGAAA
ACATTCACCCTCCGTTATCCTGAATCATTCGGGGAACGACTGACCATTGCGGCCGCTGAACGCCAAATTTCAGTGAATAC
GTTTATTGTTGAAACACTTAGCGAACACCTGAAACAGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7