Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 3385797..3386430 Replicon chromosome
Accession NZ_CP009764
Organism Ralstonia solanacearum OE1-1

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag RSOE_RS23420 Protein ID WP_016725172.1
Coordinates 3385797..3386210 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag RSOE_RS23425 Protein ID WP_011002949.1
Coordinates 3386197..3386430 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RSOE_RS23400 3381178..3382914 + 1737 WP_019718916.1 ABC transporter substrate-binding protein -
RSOE_RS23405 3383076..3384572 + 1497 WP_011002945.1 glycerol kinase GlpK -
RSOE_RS23410 3384634..3385005 - 372 WP_071507958.1 hypothetical protein -
RSOE_RS23415 3385196..3385822 + 627 WP_019718918.1 DUF1415 family protein -
RSOE_RS23420 3385797..3386210 - 414 WP_016725172.1 PIN domain-containing protein Toxin
RSOE_RS23425 3386197..3386430 - 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
RSOE_RS23430 3386487..3388094 - 1608 WP_019718919.1 glucan biosynthesis protein -
RSOE_RS23435 3388289..3389539 + 1251 WP_107523978.1 AGE family epimerase/isomerase -
RSOE_RS23440 3389547..3390842 + 1296 WP_011002952.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T49647 WP_016725172.1 NZ_CP009764:c3386210-3385797 [Ralstonia solanacearum OE1-1]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T49647 NZ_CP009764:c3386210-3385797 [Ralstonia solanacearum OE1-1]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATGCGGC
GGAAACGCTGCTGATGACCGGCGGTGTCGTCAGCGTACAGGTACTGAACGAAACGACGCACGTGATGCGCCGCAAGCTGG
CCATGCCGTGGCACGCGATCGAAACGGTGCAGGAGGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTATCGCAGAACGCTACGGATTGTCGGTCTATGACGCACTGATCGTCGCGGCGGCATTGCTTGC
CGGCTGCAACGTTCTGTATTCCGAGGATATGCAGCACGGCCTGGTGATCGAGCAGCATCTGCGCATCGTCAATCCTTTTC
CGGCACGGGCATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT49647 WP_011002949.1 NZ_CP009764:c3386430-3386197 [Ralstonia solanacearum OE1-1]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT49647 NZ_CP009764:c3386430-3386197 [Ralstonia solanacearum OE1-1]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCTATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAGCTGCACGAAGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGCGTCCGCCGCAAGCGCGCTGCGCGCGAGCTTTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References