Detailed information of TA system TA049644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 50713..51323
• Replicon: plasmid unnamed
• Accession: NZ_CP009763
• Organism: Ralstonia solanacearum OE1-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A454TTM1
• Locus tag: RSOE_RS00230
• Protein ID: WP_058907722.1
• Coordinates: 51015..51323 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RSOE_RS00225
• Protein ID: WP_016722339.1
• Coordinates: 50713..51018 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSOE_RS00200	46211..46696	+	486	WP_016726511.1	hypothetical protein	-
RSOE_RS00205	47027..47308	-	282	WP_058907724.1	hypothetical protein	-
RSOE_RS00210	47440..47724	-	285	WP_011003391.1	hypothetical protein	-
RSOE_RS25800	48029..48246	+	218	Protein_44	transposase	-
RSOE_RS00215	48413..49081	+	669	WP_071507362.1	hypothetical protein	-
RSOE_RS00220	49187..50500	-	1314	WP_071507363.1	metabolite/H+ symporter	-
RSOE_RS00225	50713..51018	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
RSOE_RS00230	51015..51323	-	309	WP_058907722.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RSOE_RS00235	51399..52598	-	1200	WP_058907721.1	MFS transporter	-
RSOE_RS00240	52725..53546	-	822	WP_011003398.1	GntR family transcriptional regulator	-
RSOE_RS00245	53724..54275	-	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
RSOE_RS00250	54408..55007	+	600	WP_071507364.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1936579	1936579

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11863.66 Da
• Isoelectric Point: 7.8930

>T49644 WP_058907722.1 NZ_CP009763:c51323-51015 [Ralstonia solanacearum OE1-1]
MLTIRTTEIFDDWFCSLRDKAAQRRIQVRIDRLQMGHPGNMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T49644 NZ_CP009763:c51323-51015 [Ralstonia solanacearum OE1-1]
ATGCTGACGATCCGCACGACCGAAATCTTCGACGACTGGTTTTGCAGCCTCCGCGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGCCACCCAGGCAACATGAAAGCGGTCCGTGACGGCATCCGTGAGCTGCGGATCG
ATCATGGGCCGGGCGACCGCTTGTACTTCGTGCGGCATGGCGTGGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT49644 WP_016722339.1 NZ_CP009763:c51018-50713 [Ralstonia solanacearum OE1-1]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT49644 NZ_CP009763:c51018-50713 [Ralstonia solanacearum OE1-1]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGATGACCCCGCCTTCATCACGCATGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACAGGGATGACCCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGGCGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Structures

Toxin

• AlphaFold DB: A0A454TTM1